Antibody data
- Antibody Data
- Antigen structure
- References [1]
- Comments [0]
- Validations
- Western blot [1]
- Immunocytochemistry [1]
- Immunohistochemistry [6]
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Validation data
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- Product number
- PA5-54523 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- HSD17B2 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant full-length protein
- Description
- Immunogen sequence: AVTMFSSVMR LELSKWGIKV ASIQPGGFLT NIAGTSDKWE KLEKDILDHL PAEVQEDYGQ DYILAQRNFL LLINSLASKD FSPVL Highest antigen sequence identity to the following orthologs: Mouse - 51%, Rat - 52%.
- Reactivity
- Human
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µL
- Concentration
- 0.1 mg/mL
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Submitted references Acetaminophen Modulates the Expression of Steroidogenesis-Associated Genes and Estradiol Levels in Human Placental JEG-3 Cells.
Addo KA, Palakodety N, Fry RC
Toxicological sciences : an official journal of the Society of Toxicology 2021 Jan 6;179(1):44-52
Toxicological sciences : an official journal of the Society of Toxicology 2021 Jan 6;179(1):44-52
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of HSD17B2 in control (vector only transfected HEK293T lysate) and HSD17B2 over-expression lysate (Co-expressed with a C-terminal myc-DDK tag (~3.1 kDa) in mammalian HEK293T cells). Samples were probed using a HSD17B2 Polyclonal Antibody (Product # PA5-54523).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent staining of HSD17B2 in human cell line RT4 using a HSD17B2 Polyclonal Antibody (Product # PA5-54523) shows localization to endoplasmic reticulum.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical staining of HSD17B2 in human duodenum and testis tissues using HSD17B2 Polyclonal Antibody (Product # PA5-54523). Corresponding HSD17B2 RNA-seq data are presented for the same tissues.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical staining of HSD17B2 in human small intestine using HSD17B2 Polyclonal Antibody (Product # PA5-54523) shows moderate to strong cytoplasmic positivity in glandular cells.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical staining of HSD17B2 in human urinary bladder using HSD17B2 Polyclonal Antibody (Product # PA5-54523) shows moderate to strong cytoplasmic positivity in urothelial cells.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical staining of HSD17B2 in human liver using HSD17B2 Polyclonal Antibody (Product # PA5-54523) shows moderate to strong cytoplasmic positivity in hepatocytes.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical staining of HSD17B2 in human duodenum using HSD17B2 Polyclonal Antibody (Product # PA5-54523) shows moderate to strong cytoplasmic positivity in glandular cells.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical staining of HSD17B2 in human testis using HSD17B2 Polyclonal Antibody (Product # PA5-54523) shows no positivity in cells in seminiferous ducts as expected.