Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [3]
- Immunohistochemistry [1]
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Validation data
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- Product number
- PA5-30152 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- RORB Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant protein fragment
- Description
- Recommended positive controls: HepG2, mouse brain. Predicted reactivity: Mouse (97%), Rat (98%), Zebrafish (86%), Chicken (96%), Sheep (96%), Rhesus Monkey (100%), Bovine (98%). Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µL
- Concentration
- 1 mg/mL
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of ROR beta using 30 µg of Neuro2A lysate. Samples were loaded onto a 10% SDS-PAGE gel and probed with a ROR beta polyclonal antibody (Product # PA5-30152) at a dilution of 1:1000.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of RORB was performed by separating 50 µg of various tissue extracts by 10% SDS-PAGE. Proteins were transferred to a membrane and probed with a RORB Polyclonal Antibody (Product # PA5-30152) at a dilution of 1:500. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot using RORB Polyclonal Antibody (Product # PA5-30152). Sample (30 µg of whole cell lysate). Lane A: HepG2. 10% SDS PAGE. RORB Polyclonal Antibody (Product # PA5-30152) diluted at 1:1,000.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry (Frozen) analysis of RORB was performed in frozen-sectioned mouse cerebral cortex tissue using RORB Polyclonal Antibody (Product # PA5-30152) at a dilution of 1:250 (Green). Blue: Fluoroshield with DAPI.