13710-1-AP

antibody from Invitrogen Antibodies

Targeting: CPE

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunoprecipitation (Data presented on provider website)

Immunohistochemistry (Supportive data in Antibodypedia)

Other assay (Supportive data in Antibodypedia)

Antibody Data

Product number

13710-1-AP

Provider

Invitrogen Antibodies

Product name

Carboxypeptidase E Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Other

Description

Immunogen sequence: FLAQYLCNE YQKGNETIVN LIHSTRIHIM PSLNPDGFEK AASQPGELKD WFVGRSNAQG IDLNRNFPDL DRIVYVNEKE GGPNNHLLKN MKKIVDQNTK LAPETKAVIH WIMDIPFVLS ANLHGGDLVA NYPYDETRSG SAHEYSSSPD DAIFQSLARA YSSFNPAMSD PNRPPCRKND DDSSFVDGTT NGGAWYSVPG GMQDFNYLSS NCFEITVELS CEKFPPEETL KTYWEDNKNS LISYLEQIHR GVKGFVRDLQ GNPIANATIS VEGIDHDVTS AKDGDYWRLL IPGNYKLTAS APGYLAITKK VAVPYSPAAG VDFELESFSE RKEEEKEELM EWWKMMSETL NF (126-476 aa encoded by BC033866)

Reactivity

Human, Mouse, Rat

Host

Rabbit

Isotype

IgG

Vial size

150 µL

Concentration

0.13 mg/mL

Storage

-20°C

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Rat brain tissue were subjected to SDS PAGE followed by western blot with 13710-1-AP (Carboxypeptidase E antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Mouse brain tissue were subjected to SDS PAGE followed by western blot with 13710-1-AP (Carboxypeptidase E antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

Immunocytochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescent analysis of ( -20°C Acetone ) fixed SH-SY5Y cells using 13710-1-AP (Carboxypeptidase E antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IGG (H+L).

Immunohistochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry of paraffin-embedded human pituitary using 13710-1-AP (Carboxypeptidase E antibody) at dilution of 1:50 (under 10x lens).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry of paraffin-embedded human pituitary using 13710-1-AP (Carboxypeptidase E antibody) at dilution of 1:50 (under 40x lens).

Other assay

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

IP result of anti-Carboxypeptidase E (IP:13710-1-AP, 3ug; Detection:13710-1-AP 1:500) with mouse brain tissue lysate 3500ug.