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Western blot
ELISAAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [2]
- Immunohistochemistry [2]
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- Product number
- LS-B11254 - Provider product page
- Provider
- LSBio
- Product name
- IHC-plus™ YARS / Tyrosyl-tRNA Synthetase Antibody LS-B11254
- Antibody type
- Polyclonal
- Description
- Caprylic acid and ammonium sulfate precipitation
- Reactivity
- Human
- Host
- Rabbit
- Isotype
- IgG
- Storage
- Aliquot and store at -20°C or -80°C. Avoid freeze-thaw cycles.
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Enhanced validation
- Submitted by
- LSBio (provider)
- Enhanced method
- Genetic validation
- Main image
- Experimental details
- Western blot of Tyrosyl-tRNA synthetase, cytoplasmic Antibody at 2 ug/ml + 293T whole cell lysate at 20 ug. Secondary: Goat polyclonal to Rabbit IgG at 1:15000 dilution. Predicted band size: 58 kDa. Observed band size:58 kDa Additional bands at: 70 kDa. We are unsure as to the identity of this extra band. This image was taken for the unconjugated form of this product. Other forms have not been tested.
- Submitted by
- LSBio (provider)
- Enhanced method
- Genetic validation
- Main image
- Experimental details
- Western blot All lanes: Tyrosyl-tRNA synthetase, cytoplasmic antibody at 2µg/ml + 293T whole cell lysate Secondary Goat polyclonal to rabbit IgG at 1/15000 dilution Predicted band size: 58 kDa Observed band size: 58 kDa
Supportive validation
- Submitted by
- LSBio (provider)
- Enhanced method
- Genetic validation
- Main image
- Experimental details
- Anti-YTS / Tyrosyl-tRNA Synthetase antibody IHC staining of human colon. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 20 ug/ml. This image was taken for the unconjugated form of this product. Other forms have not been tested.
- Submitted by
- LSBio (provider)
- Enhanced method
- Genetic validation
- Main image
- Experimental details
- Anti-YTS / Tyrosyl-tRNA Synthetase antibody IHC staining of human colon. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 20 ug/ml. This image was taken for the unconjugated form of this product. Other forms have not been tested.
