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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [1]
- Immunocytochemistry [2]
- Immunohistochemistry [8]
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- Product number
- PA5-53837 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- ERGIC1 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant full-length protein
- Description
- Immunogen sequence: VVNELYVDDP DKDSGGKIDV SLNISLPNLH CELVGLDIQD EMGRHEVGHI DNSMKIPLNN GAGCRFEGQF SINK
- Concentration
- 0.05 mg/mL
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of ERGIC1 in Lane 1: Marker (kDa) 230, 130, 95, 72, 56, 36, 28, 17, 11; Lane 2: Human cell line RT-4; Lane 3: Human cell line U-251MG sp; Lane 4: Human plasma (IgG/HSA depleted); Lane 5: Human liver tissue.; Lane 6: Human tonsil tissue. tissue. Samples were probed using an ERGIC1 Polyclonal Antibody (Product # PA5-53837).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent staining of ERGIC1 in human cell line U-251 MG shows positivity in vesicles & nucleus but excluded from the nucleoli. Samples were probed using an ERGIC1 Polyclonal Antibody (Product # PA5-53837).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent staining of ERGIC1 in human cell line U-251 MG using an ERGIC1 Polyclonal Antibody (Product # PA5-53837) shows localization to nucleoplasm and vesicles.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical staining of ERGIC1 in human cerebral cortex using ERGIC1 Polyclonal Antibody (Product # PA5-53837).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical staining of ERGIC1 in human lymph node using ERGIC1 Polyclonal Antibody (Product # PA5-53837).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical staining of ERGIC1 in human liver using ERGIC1 Polyclonal Antibody (Product # PA5-53837).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical staining of ERGIC1 in human testis using ERGIC1 Polyclonal Antibody (Product # PA5-53837).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical staining of ERGIC1 in human cerebral cortex using an ERGIC1 Polyclonal Antibody (Product # PA5-53837) shows strong cytoplasmic positivity in neuronal cells.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical staining of ERGIC1 in human cerebral cortex, liver, lymph node and testis using ERGIC1 Polyclonal Antibody (Product # PA5-53837) (A) shows similar protein distribution across tissues to an independent ERGIC1 Polyclonal Antibody (B).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical staining of ERGIC1 in human prostate using ERGIC1 Polyclonal Antibody (Product # PA5-53837) shows high expression.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical staining of ERGIC1 in human skin using ERGIC1 Polyclonal Antibody (Product # PA5-53837) shows low expression as expected.
