MAB55071

antibody from Novus Biologicals

Targeting: HMGB3 HMG2A, HMG4, MGC90319

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Data presented on provider website)

Antibody Data

Product number

MAB55071

Provider

Novus Biologicals

Product name

Mouse Monoclonal HMGB3/HMG4 Antibody

Antibody type

Monoclonal

Description

Protein A or G purified from hybridoma culture supernatant. Detects human and mouse HMGB3 in direct ELISAs and Western blots. In direct ELISAs and Western blots, no cross-reactivity with recombinant human HMG-1, A1A, A1B, or A2 is observed.

Reactivity

Human, Mouse

Host

Mouse

Isotype

IgG

Vial size

100 ug

Concentration

LYOPH

Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 degreesC as supplied. 1 month, 2 to 8 degreesC under sterile conditions after reconstitution. 6 months, -20 to -70 degreesC under sterile conditions after reconstitution.

Western blot

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Detection of Human HMGB3 by Western Blot. Western blot shows lysates of human placenta tissue. PVDF Membrane was probed with 2 µg/mL of Human/Mouse HMGB3 Monoclonal Antibody (Catalog # MAB55071) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for HMGB3 at approximately 23 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.