A300-736A

antibody from Invitrogen Antibodies

Targeting: HMGB3 HMG2A, HMG4, MGC90319

Western blot (Supportive data in Antibodypedia)

Immunoprecipitation (Recommended by provider)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

A300-736A

Provider

Invitrogen Antibodies

Product name

HMG2a Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Other

Reactivity

Human, Mouse

Host

Rabbit

Isotype

IgG

Vial size

100 µL

Concentration

0.20 mg/mL

Storage

4° C

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Detection of human HMG2a by western blot and immunoprecipitation. Samples: Whole cell lysate from HeLa (5, 15 and 50 µg for WB; 1 mg for IP, 20% of IP loaded) and 293T (T; 50 µg) cells. Antibodies: Affinity purified rabbit anti-HMG2a antibody BL3259 (Cat. No. A300-736A) used for WB at 0.04 µg/ml (A) and 1 µg/ml (B) and used for IP at 3 µg/mg lysate (B). Detection: Chemiluminescence with exposure times of 3 minutes (A) and 30 seconds (B).

Immunohistochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Detection of human and mouse HMG2a by immunohistochemistry. Sample: FFPE section of human skin basal cell carcinoma (left) and mouse squamous cell carcinoma (right). Antibody: Affinity purified rabbit anti-HMG2a (Cat. No. A300-736A Lot1) used at a dilution of 1:200 (1µg/ml). Detection: DAB.