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Western blot
ImmunocytochemistryAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Immunocytochemistry [1]
- Immunohistochemistry [1]
- Flow cytometry [3]
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- Product number
- MA5-36230 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- HMG4 Monoclonal Antibody (7G13)
- Antibody type
- Monoclonal
- Antigen
- Synthetic peptide
- Description
- Reconstitute with 0.2 mL of distilled water to yield a concentration of 500 µg/mL. Positive Control - WB: human placenta tissue, human Hela whole cell, human T-47D whole cell, human A431 whole cell, human HepG2 whole cell, human Caco-2 whole cellhuman SW620 whole cellhuman Raji whole cell. IHC: human placenta tissue. ICC/IF: Hela cell. Flow: Hela cell.|Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.
- Reactivity
- Human
- Host
- Mouse
- Isotype
- IgG
- Antibody clone number
- 7G13
- Vial size
- 100 µg
- Concentration
- 500 µg/mL
- Storage
- -20°C
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunocytochemistry analysis of HMG4 in HeLA cells. Antigen retrieval was performed with enzyme antigen retrieval (15 min). Samples were blocked with 10% goat serum and incubated in HMG4 monoclonal antibody (Product # MA5-36230) at a dilution of 2 µg/mL (overnight, 4°C), followed by Dylight 488 conjugated goat anti-mouse IgG (30 min, 37°C) and DAPI at a dilution of 5-10 µg/1x10^6 cells (30 min, 20°C).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry analysis of HMG4 in paraffin-embedded human placenta tissue. Antigen retrieval was performed with EDTA buffer (pH 8.0, epitope retrieval solution). Samples were blocked with 10% goat serum and incubated in HMG4 monoclonal antibody (Product # MA5-36230) at a dilution of 1 µg/mL (overnight, 4°C), followed by biotinylated goat anti-mouse IgG (30 min, 37°C) and Strepavidin-Biotin-Complex (SABC) with DAB.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Flow Cytometry of HMG4 in HeLa cells (blue line), isotype control mouse IgG (green line), and unlabeled (red line). Samples were blocked with 10% goat serum, incubated with HMG4 Monoclonal Antibody (7G13) (Product # MA5-36230) at a dilution of 1 μg (per 1x10^6 cells), followed by DyLight®488 conjugated goat anti-rabbit IgG (for 30 minutes at 20°C) using 5-10 μg (per 1x10^6 cells) dilution.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Flow Cytometry of HMG4 in HeLa cells (blue line), isotype control mouse IgG (green line), and unlabeled (red line). Samples were blocked with 10% goat serum, incubated with HMG4 Monoclonal Antibody (7G13) (Product # MA5-36230) at a dilution of 1 μg (per 1x10^6 cells), followed by DyLight®488 conjugated goat anti-rabbit IgG (for 30 minutes at 20°C) using 5-10 μg (per 1x10^6 cells) dilution.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Flow cytometry analysis of HMG4 in Hela cells using HMG4 Monoclonal Antibody (7G13) (Product # MA5-36230), shown in overlay histogram (blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum, and incubated with the primary antibody (1 μg/1x10^6 cells) for 30 min at 20°C. DyLight 488 conjugated goat anti-mouse IgG (5-10 µg/1x10^6 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was mouse IgG (1 µg/1x10^6) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.
