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Western blot
ImmunoprecipitationAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [2]
- Immunohistochemistry [1]
- Other assay [2]
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- Product number
- PA5-117989 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- HMGB3 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Synthetic peptide
- Reactivity
- Human
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µL
- Storage
- -20° C, Avoid Freeze/Thaw Cycles
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot using HMGB3 Polyclonal Antibody (Product # PA5-117989) at 1:500 dilution. Lane A: 293T Whole Cell Lysate, Lane B: HeLa Whole Cell Lysate. Lysates/proteins at 30 μg per lane. Secondary Goat Anti- RabbitIgG H&L (DyLight™ 800) at 1:10,000 dilution. Developed using the Odyssey technique. Performed under reducing conditions. Predicted band size: 23 kDa. Observed band size: 23 kDa.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot of HMGB3 in Lane A: 293T Whole Cell lysate, Lane B: HeLa Whole Cell Lysate. Samples (30 µg per lane) were incubated with polyclonal antibody (Product # PA5-117989) with a dilution of 1:500 , followed by Goat Anti-Rabbit IgG H&L (Dylight 800) using a dilution of 1:10,000. Assay was performed under reducing conditions. Predicted band size: 23 kDa, Observed band size: 23 kDa.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry (Paraffin) of HMGB3 in human placenta. Samples were incubated with polyclonal antibody (Product # PA5-117989) with a dilution of 1:1,500 .
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- HMGB3 Immunoprecipitation using: Lane A: 0.5 mg Jurkat Whole Cell Lysate, Lane B: 0.5 mg Hela Whole Cell Lysate 1 µL with HMGB3 Polyclonal Antibody (Product # PA5-117989) and 15 µL of 50 % Protein G agarose. Primary antibody: HMGB3 Polyclonal Antibody, at 1:500 dilution. Secondary antibody: Dylight 800-labeled antibody to rabbit IgG (H+L), at 1:5,000 dilution. Developed using the Odyssey technique. Performed under reducing conditions. Predicted band size: 23 kDa. Observed band size: 23 kDa.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunoprecipitation of HMGB3 in Lane A: 0.5 mg Jurkat Whole Cell lysate, Lane B: 0.5 mg Hela Whole Cell Lysate. Samples were treated with 15 μl of 50 % Protein G agarose, incubated with polyclonal antibody (Product # PA5-117989) with a dilution of 1:500, followed by Dylight 800-labeled antibody to rabbit IgG (H+L) using a dilution of 1:5,000. Assay was performed under reducing conditions. Predicted band size: 23 kDa, Observed band size: 23 kDa .
