15698-1-AP

antibody from Proteintech Group

Targeting: CLPP

Provider product page for 15698-1-AP

Western blot

ELISA

Immunocytochemistry

Immunoprecipitation

Immunohistochemistry

Antibody data

Antibody Data
Antigen structure
References [63]
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Validations
Western blot [1]
Immunocytochemistry [1]
Immunohistochemistry [2]

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Product number: 15698-1-AP - Provider product page
Provider: Proteintech Group
Proper citation: Proteintech Cat#15698-1-AP, RRID:AB_2245115
Product name: CLPP antibody
Antibody type: Polyclonal
Description: CLPP antibody (Cat. #15698-1-AP) is a rabbit polyclonal antibody that shows reactivity with human, mouse, rat and has been validated for the following applications: IF, IHC, IP, WB,ELISA.
Reactivity: Human, Mouse, Rat
Host: Rabbit
Conjugate: Unconjugated
Isotype: IgG
Vial size: 20ul, 150ul

CLPP protein structure - 15698-1-AP shown in red.

Submitted references Discovery of Activators of Human Caseinolytic Protease P with a Benzolactam Scaffold.

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Wogonin derivative V8 enhances bortezomib efficacy in gastric carcinoma by disrupting lysosome-mediated drug resistance.

Li SC, Shao SZ, Zhang YH, Zhou Y, Shang WT, Gao Y, He QB, Guo QL, Guo CY, Zhang XB
World journal of gastroenterology 2026 Feb 28;32(8):113299

Mitochondrial Enzymes Mimetic Ultrasmall Palladium Nanozymes Prevent Senescence and Neurodegeneration Through Metabolic Reprogramming.

Cong W, Jing H, Li Z, Zhang W, Zhang N, Xie Y, Gao S, Huang Y, Ning J
Advanced science (Weinheim, Baden-Wurttemberg, Germany) 2026 Apr 14;:e23931

ZK53 enhances tumor cell susceptibility to ferroptosis via ClpP-mediated mitochondrial dysfunction.

Dai S, Zhang T, Dai X, Zheng K, Qian H, Tong Z, Zhang Q
Frontiers in oncology 2025;15:1594840

Excessive mitochondrial stress response triggers neuronal injury through the persistent eIF2α phosphorylation in mice exposed to manganese.

Jia Y, He B, Chen K, Liu K, Yu C, Sun B, Ma Z, Xu B
Journal of hazardous materials 2025 Sep 15;496:139407

Paraquat induced neuro-immunotoxicity: Dysregulated microglial antigen processing and mitochondrial activated mechanisms.

Shi G, Wang K, Liu Q, Wu C, Zhang Q, Ma Y, Qi A, Huang M
Chemico-biological interactions 2025 Nov 1;421:111721

Dual-responsive nanoparticles targeting ACE-II senescence for therapeutic mitigation of acute lung injury.

Gao L, Zheng F, Fu Z, Wang W
Journal of nanobiotechnology 2025 May 8;23(1):339

Design, Synthesis, and Biological Evaluation of Novel Activators of Human Caseinolytic Protease P with a Pyrazololactam Scaffold.

Tang R, Li Q, Zhou H, Li Y, Li T, Jiang J, Chen Z, Kong L, Li Y, Xiao Y, Sun H
Journal of medicinal chemistry 2025 May 22;68(10):9984-10007

UPR(mt) alleviates bone cancer pain through the restoration of mitochondrial function.

Li D, Xie M, Zeng H, Yu J, Xu R, Wang Z, Huang Y, Yang Y, Sun Y
Experimental cell research 2025 May 15;448(2):114568

Mitochondrial dynamics and quality control regulate proteostasis in neuronal ischemia-reperfusion.

Fogo GM, Raghunayakula S, Emaus KJ, Torres Torres FJ, Shangguan G, Wider JM, Hüttemann M, Sanderson TH
Autophagy 2025 Jul;21(7):1492-1506

The mitochondrial DNAJC co-chaperone TCAIM reduces α-ketoglutarate dehydrogenase protein levels to regulate metabolism.

Jiahui W, Xiang Y, Youhuan Z, Xiaomin M, Yuanzhu G, Dejian Z, Jie W, Yinkun F, Shi F, Juncheng S, Masha H, Marcia H, Peiyi W, Yingjie X, Wen Y
Molecular cell 2025 Feb 6;85(3):638-651.e9

Mitochondrial protease ClpP deficiency protects against tubulointerstitial damage in diabetic kidney disease.

Wang H, Liu Y, Liu J, Zhang S, Li X, Sun L, Liu F, Liu Y, Xiao L
International immunopharmacology 2025 Dec 3;166:115641

LONP1 regulation of mitochondrial protein folding provides insight into beta cell failure in type 2 diabetes.

Li J, Deng Y, Gasser M, Zhu J, Walker EM, Sidarala V, Reck EC, Hubers DL, Pasmooij MB, Shin CS, Bandesh K, Motakis E, Nargund S, Kursawe R, Basrur V, Nesvizhskii AI, Stitzel ML, Chan DC, Rutter GA, Soleimanpour SA
Nature metabolism 2025 Aug;7(8):1570-1592

Atrazine exposure induces TDP-43 protein translocation: A potential mechanism for prefrontal cortical neurodegeneration induced by environmental pollutants.

Ma Y, Wang K, Jiao Y, Li Y, Hu R, Li Y, Shi G, Huang M
Toxicology 2025 Aug;515:154128

Leber's hereditary optic neuropathy-associated ND1 3733G>C mutation ameliorates the mitochondrial quality control and cellular homeostasis.

Yasheng M, Ji Y, He Y, Yi Q, Zhang H, Shan W, Wang K, Zhang J, Li Y, Meng F, Zhang M, Mo JQ, Wei S, Guan MX
The Journal of biological chemistry 2025 Aug;301(8):110464

ONC206 targeting ClpP induces mitochondrial dysfunction and protective autophagy in hepatocellular carcinoma cells.

Cao J, Cao F, Wang C, Jiao Z, You Y, Wang X, Zhao W
Neoplasia (New York, N.Y.) 2024 Sep;55:101015

Discovery of Dehydrogenated Imipridone Derivatives as Activators of Human Caseinolytic Protease P.

Jiang J, Xie G, Li T, Ding H, Tang R, Luo J, Li Q, Lu W, Xiao Y, Sun H
Journal of medicinal chemistry 2024 Sep 12;67(17):15328-15352

Mitophagy Facilitates Cytosolic Proteostasis to Preserve Cardiac Function.

Rawnsley DR, Islam M, Zhao C, Kargar Gaz Kooh Y, Mendoza A, Navid H, Kumari M, Guan X, Murphy JT, Nigro J, Kovacs A, Mani K, Huebsch N, Ma X, Diwan A
bioRxiv : the preprint server for biology 2024 Nov 26;

The mitochondrial UPR induced by ATF5 attenuates intervertebral disc degeneration via cooperating with mitophagy.

Xu WN, Zheng HL, Yang RZ, Sun YF, Peng BR, Liu C, Song J, Jiang SD, Zhu LX
Cell biology and toxicology 2024 Mar 13;40(1):16

Caseinolytic mitochondrial matrix peptidase X is essential for homologous chromosome synapsis and recombination during meiosis of male mouse germ cells.

Feng HW, Zhao Y, Gao YL, Liu DT, Huo LJ
Asian journal of andrology 2024 Mar 1;26(2):165-174

Zearalenone modulates the function of goat endometrial cells via the mitochondrial quality control system.

Zhang G, Zeng C, Sun X, Zhang Q, Wang Y, Xia R, Mai Q, Xue G, Huang H, Wang F
FASEB journal : official publication of the Federation of American Societies for Experimental Biology 2024 Jul 15;38(13):e23701

CTRP3 alleviates mitochondrial dysfunction and oxidative stress injury in pathological cardiac hypertrophy by activating UPRmt via the SIRT1/ATF5 axis.

Shi L, Tan Y, Zheng W, Cao G, Zhou H, Li P, Cui J, Song Y, Feng L, Li H, Shan W, Zhang B, Yi W
Cell death discovery 2024 Jan 26;10(1):53

Mic19 depletion impairs endoplasmic reticulum-mitochondrial contacts and mitochondrial lipid metabolism and triggers liver disease.

Dong J, Chen L, Ye F, Tang J, Liu B, Lin J, Zhou PH, Lu B, Wu M, Lu JH, He JJ, Engelender S, Meng Q, Song Z, He H
Nature communications 2024 Jan 2;15(1):168

Stress granules affect the dual PI3K/mTOR inhibitor response by regulating the mitochondrial unfolded protein response.

Lin N, Sun L, Chai J, Qi H, Zhao Y, Ma J, Xia M, Hu X
Cancer cell international 2024 Jan 18;24(1):38

CLPP-Null Eukaryotes with Excess Heme Biosynthesis Show Reduced L-arginine Levels, Probably via CLPX-Mediated OAT Activation.

Key J, Gispert S, Kandi AR, Heinz D, Hamann A, Osiewacz HD, Meierhofer D, Auburger G
Biomolecules 2024 Feb 19;14(2)

Development of novel imipridone derivatives with potent anti-cancer activities as human caseinolytic peptidase P (hClpP) activators.

Zhang Y, Jiang J, Ding H, Li Q, Xiao Y, Sun H
Bioorganic chemistry 2024 Dec;153:107765

Dietary cobalt oxide nanoparticles alleviate aging through activation of mitochondrial UPR in Caenorhabditis elegans.

Cong W, Wang Y, Yuan C, Xu M, Wang H, Hu Y, Dai X, Weng Y, Timashev P, Liang XJ, Huang Y
Theranostics 2023;13(10):3276-3289

Translation Fidelity and Respiration Deficits in CLPP-Deficient Tissues: Mechanistic Insights from Mitochondrial Complexome Profiling.

Key J, Gispert S, Koepf G, Steinhoff-Wagner J, Reichlmeir M, Auburger G
International journal of molecular sciences 2023 Dec 15;24(24)

Leber's hereditary optic neuropathy-associated ND6 14484T > C mutation caused pleiotropic effects on the complex I, RNA homeostasis, apoptosis and mitophagy.

Liang M, Ji Y, Zhang L, Wang X, Hu C, Zhang J, Zhu Y, Mo JQ, Guan MX
Human molecular genetics 2022 Sep 29;31(19):3299-3312

Lysosomal damage drives mitochondrial proteome remodelling and reprograms macrophage immunometabolism.

Bussi C, Heunis T, Pellegrino E, Bernard EM, Bah N, Dos Santos MS, Santucci P, Aylan B, Rodgers A, Fearns A, Mitschke J, Moore C, MacRae JI, Greco M, Reinheckel T, Trost M, Gutierrez MG
Nature communications 2022 Nov 28;13(1):7338

KPNB1 modulates the Machado-Joseph disease protein ataxin-3 through activation of the mitochondrial protease CLPP.

Abeditashi M, Weber JJ, Pereira Sena P, Velic A, Kalimeri M, Incebacak Eltemur RD, Schmidt J, Hübener-Schmid J, Hauser S, Macek B, Riess O, Schmidt T
Cellular and molecular life sciences : CMLS 2022 Jul 6;79(8):401

Mitochondrial proteostasis stress in muscle drives a long-range protective response to alleviate dietary obesity independently of ATF4.

Guo Q, Xu Z, Zhou D, Fu T, Wang W, Sun W, Xiao L, Liu L, Ding C, Yin Y, Zhou Z, Sun Z, Zhu Y, Zhou W, Jia Y, Xue J, Chen Y, Chen XW, Piao HL, Lu B, Gan Z
Science advances 2022 Jul 29;8(30):eabo0340

Disuse-associated loss of the protease LONP1 in muscle impairs mitochondrial function and causes reduced skeletal muscle mass and strength.

Xu Z, Fu T, Guo Q, Zhou D, Sun W, Zhou Z, Chen X, Zhang J, Liu L, Xiao L, Yin Y, Jia Y, Pang E, Chen Y, Pan X, Fang L, Zhu MS, Fei W, Lu B, Gan Z
Nature communications 2022 Feb 16;13(1):894

CLPP Depletion Causes Diplotene Arrest; Underlying Testis Mitochondrial Dysfunction Occurs with Accumulation of Perrault Proteins ERAL1, PEO1, and HARS2.

Key J, Gispert S, Koornneef L, Sleddens-Linkels E, Kohli A, Torres-Odio S, Koepf G, Amr S, Reichlmeir M, Harter PN, West AP, Münch C, Baarends WM, Auburger G
Cells 2022 Dec 22;12(1)

Balanced mitochondrial and cytosolic translatomes underlie the biogenesis of human respiratory complexes.

Soto I, Couvillion M, Hansen KG, McShane E, Moran JC, Barrientos A, Churchman LS
Genome biology 2022 Aug 9;23(1):170

A Mitochondrial DNA Variant Elevates the Risk of Gallstone Disease by Altering Mitochondrial Function.

Sun D, Niu Z, Zheng HX, Wu F, Jiang L, Han TQ, Wei Y, Wang J, Jin L
Cellular and molecular gastroenterology and hepatology 2021;11(4):1211-1226.e15

Proteomic analysis demonstrates the role of the quality control protease LONP1 in mitochondrial protein aggregation.

Pollecker K, Sylvester M, Voos W
The Journal of biological chemistry 2021 Oct;297(4):101134

Increased presence of nuclear DNAJA3 and upregulation of cytosolic STAT1 and of nucleic acid sensors trigger innate immunity in the ClpP-null mouse.

Maletzko A, Key J, Wittig I, Gispert S, Koepf G, Canet-Pons J, Torres-Odio S, West AP, Auburger G
Neurogenetics 2021 Oct;22(4):297-312

Multi-OMICS study of a CHCHD10 variant causing ALS demonstrates metabolic rewiring and activation of endoplasmic reticulum and mitochondrial unfolded protein responses.

Straub IR, Weraarpachai W, Shoubridge EA
Human molecular genetics 2021 May 17;30(8):687-705

Parkin-independent mitophagy via Drp1-mediated outer membrane severing and inner membrane ubiquitination.

Oshima Y, Cartier E, Boyman L, Verhoeven N, Polster BM, Huang W, Kane M, Lederer WJ, Karbowski M
The Journal of cell biology 2021 Jun 7;220(6)

Prohibitin 1 is essential to preserve mitochondria and myelin integrity in Schwann cells.

Della-Flora Nunes G, Wilson ER, Marziali LN, Hurley E, Silvestri N, He B, O'Malley BW, Beirowski B, Poitelon Y, Wrabetz L, Feltri ML
Nature communications 2021 Jun 2;12(1):3285

An animal model for mitochondrial tyrosyl-tRNA synthetase deficiency reveals links between oxidative phosphorylation and retinal function.

Jin X, Zhang Z, Nie Z, Wang C, Meng F, Yi Q, Chen M, Sun J, Zou J, Jiang P, Guan MX
The Journal of biological chemistry 2021 Jan-Jun;296:100437

SIRT3-mediated mitochondrial unfolded protein response weakens breast cancer sensitivity to cisplatin.

Chen H, Zhang DM, Zhang ZP, Li MZ, Wu HF
Genes & genomics 2021 Dec;43(12):1433-1444

Loss of Mitochondrial Protease CLPP Activates Type I IFN Responses through the Mitochondrial DNA-cGAS-STING Signaling Axis.

Torres-Odio S, Lei Y, Gispert S, Maletzko A, Key J, Menissy SS, Wittig I, Auburger G, West AP
Journal of immunology (Baltimore, Md. : 1950) 2021 Apr 15;206(8):1890-1900

Nicotinamide Riboside Enhances Mitochondrial Proteostasis and Adult Neurogenesis through Activation of Mitochondrial Unfolded Protein Response Signaling in the Brain of ALS SOD1(G93A) Mice.

Zhou Q, Zhu L, Qiu W, Liu Y, Yang F, Chen W, Xu R
International journal of biological sciences 2020;16(2):284-297

Mitochondrial DNA Haplogroup M7 Confers Disability in a Chinese Aging Population.

Sun D, Yao S, Wu F, Deng W, Ma Y, Jin L, Wang J, Wang X
Frontiers in genetics 2020;11:577795

Mutant huntingtin does not cross the mitochondrial outer membrane.

Hamilton J, Brustovetsky T, Khanna R, Brustovetsky N
Human molecular genetics 2020 Oct 10;29(17):2962-2975

Asymmetrical effects of deafness-associated mitochondrial DNA 7516delA mutation on the processing of RNAs in the H-strand and L-strand polycistronic transcripts.

Xiao Y, Wang M, He Q, Xu L, Zhang Q, Meng F, Jia Z, Zhang F, Wang H, Guan MX
Nucleic acids research 2020 Nov 4;48(19):11113-11129

Overexpression of mitochondrial histidyl-tRNA synthetase restores mitochondrial dysfunction caused by a deafness-associated tRNA(His) mutation.

Gong S, Wang X, Meng F, Cui L, Yi Q, Zhao Q, Cang X, Cai Z, Mo JQ, Liang Y, Guan MX
The Journal of biological chemistry 2020 Jan 24;295(4):940-954

Mitochondrial serine protease Omi/HtrA2 accentuates brain ischemia/reperfusion injury in rats and oxidative stress injury in vitro by modulating mitochondrial stress proteins CHOP and ClpP and physically interacting with mitochondrial fusion protein OPA1.

You H, Jin Y, Kang J, Mao Y, Su J, Sun L, Wang L, Meng H
Bioengineered 2020 Dec;11(1):1058-1070

TDP-43 induces mitochondrial damage and activates the mitochondrial unfolded protein response.

Wang P, Deng J, Dong J, Liu J, Bigio EH, Mesulam M, Wang T, Sun L, Wang L, Lee AY, McGee WA, Chen X, Fushimi K, Zhu L, Wu JY
PLoS genetics 2019 May;15(5):e1007947

Deletion of Gtpbp3 in zebrafish revealed the hypertrophic cardiomyopathy manifested by aberrant mitochondrial tRNA metabolism.

Chen D, Zhang Z, Chen C, Yao S, Yang Q, Li F, He X, Ai C, Wang M, Guan MX
Nucleic acids research 2019 Jun 4;47(10):5341-5355

Caloric Restriction Induces MicroRNAs to Improve Mitochondrial Proteostasis.

Zhang R, Wang X, Qu JH, Liu B, Zhang P, Zhang T, Fan PC, Wang XM, Xiao GY, Su Y, Xie Y, Liu Y, Pei JF, Zhang ZQ, Hao DL, Xu P, Chen HZ, Liu DP
iScience 2019 Jul 26;17:155-166

Fibroblast Growth Factor 21 Drives Dynamics of Local and Systemic Stress Responses in Mitochondrial Myopathy with mtDNA Deletions.

Forsström S, Jackson CB, Carroll CJ, Kuronen M, Pirinen E, Pradhan S, Marmyleva A, Auranen M, Kleine IM, Khan NA, Roivainen A, Marjamäki P, Liljenbäck H, Wang L, Battersby BJ, Richter U, Velagapudi V, Nikkanen J, Euro L, Suomalainen A
Cell metabolism 2019 Dec 3;30(6):1040-1054.e7

LONP1 Is Required for Maturation of a Subset of Mitochondrial Proteins, and Its Loss Elicits an Integrated Stress Response.

Zurita Rendón O, Shoubridge EA
Molecular and cellular biology 2018 Oct 15;38(20)

Sam50 Regulates PINK1-Parkin-Mediated Mitophagy by Controlling PINK1 Stability and Mitochondrial Morphology.

Jian F, Chen D, Chen L, Yan C, Lu B, Zhu Y, Chen S, Shi A, Chan DC, Song Z
Cell reports 2018 Jun 5;23(10):2989-3005

mTORC1 Regulates Mitochondrial Integrated Stress Response and Mitochondrial Myopathy Progression.

Khan NA, Nikkanen J, Yatsuga S, Jackson C, Wang L, Pradhan S, Kivelä R, Pessia A, Velagapudi V, Suomalainen A
Cell metabolism 2017 Aug 1;26(2):419-428.e5

Muscle mitochondrial stress adaptation operates independently of endogenous FGF21 action.

Ost M, Coleman V, Voigt A, van Schothorst EM, Keipert S, van der Stelt I, Ringel S, Graja A, Ambrosi T, Kipp AP, Jastroch M, Schulz TJ, Keijer J, Klaus S
Molecular metabolism 2016 Feb;5(2):79-90

Quality control of mitochondrial protein synthesis is required for membrane integrity and cell fitness.

Richter U, Lahtinen T, Marttinen P, Suomi F, Battersby BJ
The Journal of cell biology 2015 Oct 26;211(2):373-89

Effective treatment of mitochondrial myopathy by nicotinamide riboside, a vitamin B3.

Khan NA, Auranen M, Paetau I, Pirinen E, Euro L, Forsström S, Pasila L, Velagapudi V, Carroll CJ, Auwerx J, Suomalainen A
EMBO molecular medicine 2014 Jun;6(6):721-31

Proteolytic cleavage of Opa1 stimulates mitochondrial inner membrane fusion and couples fusion to oxidative phosphorylation.

Mishra P, Carelli V, Manfredi G, Chan DC
Cell metabolism 2014 Apr 1;19(4):630-41

Loss of mitochondrial peptidase Clpp leads to infertility, hearing loss plus growth retardation via accumulation of CLPX, mtDNA and inflammatory factors.

Gispert S, Parganlija D, Klinkenberg M, Dröse S, Wittig I, Mittelbronn M, Grzmil P, Koob S, Hamann A, Walter M, Büchel F, Adler T, Hrabé de Angelis M, Busch DH, Zell A, Reichert AS, Brandt U, Osiewacz HD, Jendrach M, Auburger G
Human molecular genetics 2013 Dec 15;22(24):4871-87

The transcription factor C/EBP delta has anti-apoptotic and anti-inflammatory roles in pancreatic beta cells.

Moore F, Santin I, Nogueira TC, Gurzov EN, Marselli L, Marchetti P, Eizirik DL
PloS one 2012;7(2):e31062

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Supportive validation

Submitted by: Proteintech Group (provider)
Main image
Experimental details: K-562 cells were subjected to SDS PAGE followed by western blot with 15698-1-AP(CLPP antibody) at dilution of 1:500
Sample type: cell line

Supportive validation

Submitted by: Proteintech Group (provider)
Main image
Experimental details: Immunofluorescent analysis of Hela cells, using CLPP antibody 15698-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).
Sample type: cell line

Supportive validation

Submitted by: Proteintech Group (provider)
Main image
Experimental details: Immunohistochemical of paraffin-embedded human heart using 15698-1-AP(CLPP antibody) at dilution of 1:50 (under 10x lens)
Sample type: tissue

Supportive validation

Submitted by: Proteintech Group (provider)
Main image
Experimental details: Immunohistochemical of paraffin-embedded human heart using 15698-1-AP(CLPP antibody) at dilution of 1:50 (under 40x lens)
Sample type: tissue