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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [3]
- Immunocytochemistry [1]
- Immunohistochemistry [1]
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- Product number
- GTX104656 - Provider product page
- Provider
- GeneTex
- Proper citation
- GeneTex Cat#GTX104656, RRID:AB_1240636
- Product name
- CLPP antibody [C2C3], C-term
- Antibody type
- Polyclonal
- Reactivity
- Human
- Host
- Rabbit
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Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Sample(30 £gg of whole cell lysate)A:293TB:A431(GTX27909) C:H129910% SDS PAGEGTX104656 diluted at 1:1500
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- CLPP antibody detects CLPP protein by western blot analysis. Various whole cell extracts (30 £gg) were separated by 12% SDS-PAGE, and the membrane was blotted with CLPP antibody (GTX104656) diluted by 1:500.
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Wild-type (WT) and CLPP knockout (KO) 293T cell extracts (30 ?g) were separated by 12% SDS-PAGE, and the membrane was blotted with CLPP antibody [C2C3], C-term (GTX104656) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- CLPP antibody [C2C3], C-term detects CLPP protein at mitochondria by immunofluorescent analysis.Sample: A431 cells were fixed in 2% paraformaldehyde/culture medium at RT for 30 min.Green: CLPP protein stained by CLPP antibody [C2C3], C-term (GTX104656) diluted at 1:500.Blue: Hoechst 33342 staining.Scale bar = 10 £gm.
Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of paraffin-embedded H520 xenograft, using CLPP(GTX104656) antibody at 1:100 dilution.
