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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [2]
- Immunocytochemistry [1]
- Immunohistochemistry [1]
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- Product number
- PA5-21591 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- CLPP Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Synthetic peptide
- Description
- Recommended positive controls: 293T, Jurkat, Raji, NCI-H929.
- Concentration
- 1 mg/mL
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of ClpP using 30 µg of A) 293T (B) A431 and C) H1299 lysate. Samples were loaded onto a 10% SDS-PAGE gel and probed with a ClpP polyclonal antibody (Product # PA5-21591) at a dilution of 1:1500.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- CLPP antibody detects CLPP protein by western blot analysis. Various whole cell extracts (30 µg) were separated by 12% SDS-PAGE, and the membrane was blotted with CLPP antibody CLPP Polyclonal Antibody (Product # PA5-21591) diluted by 1:500.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunocytochemistry-Immunofluorescence analysis of CLPP was performed in A431 cells fixed in 2% paraformaldehyde/culture medium at RT for 30 min. Green: CLPP Polyclonal Antibody (Product # PA5-21591) diluted at 1:500. Blue: Hoechst 33342 staining. Scale bar = 10 µm.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of paraffin-embedded H520 xenograft, using CLPP (Product # PA5-21591) antibody at 1:100 dilution. Antigen Retrieval: EDTA based buffer, pH 8.0, 15 min.
