HPA010649

antibody from Atlas Antibodies

Targeting: CLPP

Western blot (Enhanced validation)

Immunocytochemistry (Data presented on provider website)

Immunohistochemistry (Data presented on provider website)

Antibody Data

Product number

HPA010649

Provider

Atlas Antibodies

Proper citation

Atlas Antibodies Cat#HPA010649, RRID:AB_1078538

Product name

Anti-CLPP

Antibody type

Polyclonal

Description

Polyclonal Antibody against Human CLPP, Gene description: caseinolytic mitochondrial matrix peptidase proteolytic subunit, Validated applications: IHC, ICC, WB, Uniprot ID: Q16740, Storage: Store at +4°C for short term storage. Long time storage is recommended at -20°C.

Reactivity

Human, Mouse, Rat

Host

Rabbit

Conjugate

Unconjugated

Isotype

IgG

Vial size

100 µl

Concentration

0.1 mg/ml

Storage

Store at +4°C for short term storage. Long time storage is recommended at -20°C.

Handling

The antibody solution should be gently mixed before use.

References

Submitted references

Parkinson’s disease neurons exhibit alterations in mitochondrial quality control proteins

Chen C, McDonald D, Blain A, Mossman E, Atkin K, Marusich M, Capaldi R, Bone L, Smith A, Filby A, Erskine D, Russell O, Hudson G, Vincent A, Reeve A
npj Parkinson's Disease 2023;9(1)

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Dietary apple polyphenols enhance mitochondrial turnover and respiratory chain enzymes

Yoshida Y, Tamura Y, Kouzaki K, Nakazato K
Experimental Physiology 2023;108(10):1295-1307

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Mitochondria regulate intracellular coenzyme Q transport and ferroptotic resistance via STARD7

Deshwal S, Onishi M, Tatsuta T, Bartsch T, Cors E, Ried K, Lemke K, Nolte H, Giavalisco P, Langer T
Nature Cell Biology 2023

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The Mouse Heart Mitochondria N Terminome Provides Insights into ClpXP-Mediated Proteolysis

Hofsetz E, Demir F, Szczepanowska K, Kukat A, Kizhakkedathu J, Trifunovic A, Huesgen P
Molecular & Cellular Proteomics 2020;19(8):1330-1345

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Subcellular origin of mitochondrial DNA deletions in human skeletal muscle

Vincent A, Rosa H, Pabis K, Lawless C, Chen C, Grünewald A, Rygiel K, Rocha M, Reeve A, Falkous G, Perissi V, White K, Davey T, Petrof B, Sayer A, Cooper C, Deehan D, Taylor R, Turnbull D, Picard M
Annals of Neurology 2018;84(2):289-301

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Perrault Syndrome Is Caused by Recessive Mutations in CLPP, Encoding a Mitochondrial ATP-Dependent Chambered Protease

Jenkinson E, Rehman A, Walsh T, Clayton-Smith J, Lee K, Morell R, Drummond M, Khan S, Naeem M, Rauf B, Billington N, Schultz J, Urquhart J, Lee M, Berry A, Hanley N, Mehta S, Cilliers D, Clayton P, Kingston H, Smith M, Warner T, Black G, Trump D, Davis J, Ahmad W, Leal S, Riazuddin S, King M, Friedman T, Newman W
The American Journal of Human Genetics 2013;92(4):605-613

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Systematic validation of antibody binding and protein subcellular localization using siRNA and confocal microscopy

Stadler C, Hjelmare M, Neumann B, Jonasson K, Pepperkok R, Uhlén M, Lundberg E
Journal of Proteomics 2012;75(7):2236-2251

Western blot

Enhanced validation

Submitted by

Atlas Antibodies (provider)

Enhanced method

Genetic validation

Main image

Experimental details

Western blot analysis in HEK293 cells transfected with control siRNA, target specific siRNA probe #1, using Anti-CLPP antibody. Remaining relative intensity is presented. Loading control: Anti-PPIB.

Sample type

Human

Protocol

Protocol