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LearnPA5-22235
antibody from Invitrogen Antibodies
Targeting: CNGA2
CNCA, CNCA1, CNG2, FLJ46312, OCNC1, OCNCa, OCNCALPHA
Western blotAntibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [2]
- Immunocytochemistry [1]
- Immunohistochemistry [1]
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- Product number
- PA5-22235 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- CNGA2 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant protein fragment
- Description
- Recommended positive controls: 293T, A431, H1299, HeLa, HepG2, mouse brain. Predicted reactivity: Mouse (97%), Rat (96%), Rabbit (97%), Bovine (96%). Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.
- Reactivity
- Human, Mouse
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µL
- Concentration
- 1 mg/mL
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot analysis of CNGA2 was performed by separating 50 µg of Mouse tissue extracts by 7.5% SDS-PAGE. Proteins were transferred to a membrane and probed with a CNGA2 Polyclonal Antibody (Product # PA5-22235) at a dilution of 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot using CNGA2 Polyclonal Antibody (Product # PA5-22235). Sample (30 µg of whole cell lysate). Lane A: 293T. Lane B: A431. Lane C: Hep G2 . 7.5% SDS PAGE. CNGA2 Polyclonal Antibody (Product # PA5-22235) diluted at 1:1,000.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent analysis of CNGA2 in methanol-fixed HeLa cells using a CNGA2 polyclonal antibody (Product # PA5-22235) at a 1:200 dilution.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of paraffin-embedded SG xenograft , using CNGA2 (Product # PA5-22235) antibody at 1:500 dilution. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.
