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LearnMA5-26118
antibody from Invitrogen Antibodies
Targeting: MAGEA4
CT1.4, MAGE-41, MAGE-X2, MAGE4, MAGE4A, MAGE4B, MGC21336
Western blotAntibody data
- Antibody Data
- Antigen structure
- References [1]
- Comments [0]
- Validations
- Western blot [1]
- Immunohistochemistry [1]
- Other assay [1]
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- Product number
- MA5-26118 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- MAGEA4 Monoclonal Antibody (OTI1F9)
- Antibody type
- Monoclonal
- Antigen
- Recombinant full-length protein
- Reactivity
- Human
- Host
- Mouse
- Isotype
- IgG
- Antibody clone number
- OTI1F9
- Vial size
- 100 µL
- Concentration
- 1 mg/mL
- Storage
- -20° C, Avoid Freeze/Thaw Cycles
Submitted references Development of a CD8 co-receptor independent T-cell receptor specific for tumor-associated antigen MAGE-A4 for next generation T-cell-based immunotherapy.
Davari K, Holland T, Prassmayer L, Longinotti G, Ganley KP, Pechilis LJ, Diaconu I, Nambiar PR, Magee MS, Schendel DJ, Sommermeyer D, Ellinger C
Journal for immunotherapy of cancer 2021 Mar;9(3)
Journal for immunotherapy of cancer 2021 Mar;9(3)
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of MAGEA4 in HEK293T cells in untransfected (Left lane) and transfected (Right lane) samples using 5 µg per lane. The samples were separated by SDS-PAGE and probed with MAGEA4 (Product # MA5-26118) monoclonal antibody.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry was performed on paraffin-embedded carcinoma of human bladder tissue. To expose target proteins, 10mM citric buffer, pH6.0, 120°C for 3min was used. Following antigen retrieval, tissues were probed with a MAGEA4 monoclonal antibody (Product # MA5-26118).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Figure 1 Tumor-associated antigen MAGE-A4 harbors an immunogenic candidate T-cell epitope. (A) Formalin-fixed paraffin-embedded human tumors of different histologic subtype (TNBC n=62, HNSCC n=172, NSCLC n=158, ovarian n=89) were stained with an anti-human MAGE-A4 antibody and evaluated by a study pathologist. The intensity of staining was scored based on a 3-point scale, the percentages of cells assigned to each score were estimated, and an H-score was assigned. Numbers below graph indicate the percentage of samples with an H-score >=100. (B) CD8 + T cells from an HLA-A*02:01-positive healthy donor were expanded for 12 days by stimulation with autologous monocyte-derived dendritic cells electroporated with ivt-RNA encoding for MAGE-A4. Left dot plot depicts the gating strategy of expanded CD8 + T cells 16 hours after co-culture with MAGE-A4-negative A2+K562 cells for CD137-negative bulk sorting. CD137-negative sorted cells were co-cultured with A2+K562 cells transduced with MAGE-A4; 16 hours later, CD137-positive cells (right) were single cell sorted. (C, D) Single cell sort-derived T-cell clones were expanded for 15 days and tested for reactivity toward MAGE-A4. Shown are bar graphs displaying IFN-gamma concentrations in supernatants of two positive T-cell clones 16 hours after co-culture with target cells for screening. (C) Co-culture with A2+K562 cells transduced with MAGE-A4 or an irrelevant protein (irrel. prot.). (D) Co-culture with A2+K562 cells pulsed with different
