MA5-26118

antibody from Invitrogen Antibodies

Targeting: MAGEA4 CT1.4, MAGE-41, MAGE-X2, MAGE4, MAGE4A, MAGE4B, MGC21336

Western blot (Data presented on provider website)

Immunohistochemistry (Data presented on provider website)

Flow cytometry (Recommended by provider)

Other assay (Supportive data in Antibodypedia)

Antibody Data

Product number

MA5-26118

Provider

Invitrogen Antibodies

Product name

MAGEA4 Monoclonal Antibody (OTI1F9)

Antibody type

Monoclonal

Antigen

Recombinant full-length protein

Reactivity

Human

Host

Mouse

Isotype

IgG

Antibody clone number

OTI1F9

Vial size

100 µL

Concentration

1 mg/mL

Storage

-20° C, Avoid Freeze/Thaw Cycles

References

Submitted references

Development of a CD8 co-receptor independent T-cell receptor specific for tumor-associated antigen MAGE-A4 for next generation T-cell-based immunotherapy.

Davari K, Holland T, Prassmayer L, Longinotti G, Ganley KP, Pechilis LJ, Diaconu I, Nambiar PR, Magee MS, Schendel DJ, Sommermeyer D, Ellinger C
Journal for immunotherapy of cancer 2021 Mar;9(3)

Other assay

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Figure 1 Tumor-associated antigen MAGE-A4 harbors an immunogenic candidate T-cell epitope. (A) Formalin-fixed paraffin-embedded human tumors of different histologic subtype (TNBC n=62, HNSCC n=172, NSCLC n=158, ovarian n=89) were stained with an anti-human MAGE-A4 antibody and evaluated by a study pathologist. The intensity of staining was scored based on a 3-point scale, the percentages of cells assigned to each score were estimated, and an H-score was assigned. Numbers below graph indicate the percentage of samples with an H-score >=100. (B) CD8 + T cells from an HLA-A*02:01-positive healthy donor were expanded for 12 days by stimulation with autologous monocyte-derived dendritic cells electroporated with ivt-RNA encoding for MAGE-A4. Left dot plot depicts the gating strategy of expanded CD8 + T cells 16 hours after co-culture with MAGE-A4-negative A2+K562 cells for CD137-negative bulk sorting. CD137-negative sorted cells were co-cultured with A2+K562 cells transduced with MAGE-A4; 16 hours later, CD137-positive cells (right) were single cell sorted. (C, D) Single cell sort-derived T-cell clones were expanded for 15 days and tested for reactivity toward MAGE-A4. Shown are bar graphs displaying IFN-gamma concentrations in supernatants of two positive T-cell clones 16 hours after co-culture with target cells for screening. (C) Co-culture with A2+K562 cells transduced with MAGE-A4 or an irrelevant protein (irrel. prot.). (D) Co-culture with A2+K562 cells pulsed with different