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Learn12508-1-AP
antibody from Invitrogen Antibodies
Targeting: MAGEA4
CT1.4, MAGE-41, MAGE-X2, MAGE4, MAGE4A, MAGE4B, MGC21336
Western blotAntibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [5]
- Immunohistochemistry [1]
- Flow cytometry [1]
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- Product number
- 12508-1-AP - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- MAGEA4 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Other
- Description
- Immunogen sequence: MSSEQKSQH CKPEEGVEAQ EEALGLVGAQ APTTEEQEAA VSSSSPLVPG TLEEVPAAES AGPPQSPQGA SALPTTISFT CWRQPNEGSS SQEEEGPSTS PDAESLFREA LSNKVDELAH FLLRKYRAKE LVTKAEMLER VIKNYKRCFP VIFGKASESL KMIFGIDVKE VDPTSNTYTL VTCLGLSYDG LLGNNQIFPK TGLLIIVLGT IAMEGDSASE EEIWEELGVM GVYDGREHTV YGEPRKLLTQ DWVQENYLEY RQVPGSNPAR YEFLWGPRAL AETSYVKVLE HVVRVNARVR IAYPSLREAA LLEEEEGV (1-317 aa encoded by BC017723)
- Reactivity
- Human
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 150 µL
- Concentration
- 0.17 mg/mL
- Storage
- -20°C
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- A431 cells were subjected to SDS PAGE followed by western blot with 12508-1-AP (MAGEA4 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- U2OS cells were subjected to SDS PAGE followed by western blot with 12508-1-AP (MAGEA4 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- A431 cells were subjected to SDS PAGE followed by western blot with 12508-1-AP (MAGEA4 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- U2OS cells were subjected to SDS PAGE followed by western blot with 12508-1-AP (MAGEA4 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Transfected HEK-293 cells were subjected to SDS PAGE followed by western blot with 12508-1-AP ( MAGEA4 Antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry of paraffin-embedded human breast cancer using 12508-1-AP (MAGEA4 antibody) at dilution of 1:50 (under 10x lens).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- 1X10^6 A431 cells were stained with 0.2 µg Anti-Human MAGEA4 (Product # 12508-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1,000 (green), or stained with 0.2 µg isotype control and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1,000 (black). Cells were fixed with 90% MeOH.
