Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [2]
- Immunohistochemistry [1]
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Validation data
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- Product number
- AP2131a - Provider product page
- Provider
- Abcepta
- Proper citation
- Abgent Cat#AP2131a, RRID:AB_2212430
- Product name
- USP2 Antibody (N-term)
- Antibody type
- Polyclonal
- Antigen
- Synthetic peptide
- Description
- Purified Rabbit Polyclonal Antibody (Pab)
- Reactivity
- Human
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 400 µl
- Concentration
- 2 mg/ml
- Storage
- Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
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Supportive validation
- Submitted by
- Abcepta (provider)
- Main image
- Experimental details
- Western blot analysis of USP2 Antibody (N-term) (Cat. #AP2131a) in HL60 cell line lysates (35ug/lane). USP2 (arrow) was detected using the purified Pab.
- Primary Ab dilution
- 1:1000
- Submitted by
- Abcepta (provider)
- Main image
- Experimental details
- Western blot analysis of USP2 (arrow) using rabbit polyclonal USP2 Antibody (T22) (Cat. #AP2131a). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the USP2 gene.
- Primary Ab dilution
- 1:1000
Supportive validation
- Submitted by
- Abcepta (provider)
- Main image
- Experimental details
- "IHC for USP2a in a formalin-fixed, paraffin-embedded human prostate sample. Cytoplasmic positive immunostaining was detected in tumor glands (ii), whereas in normal prostatic epithelium, USP2a expression was restricted to the basal layer."
- Primary Ab dilution
- 1:10~50