Antibody data
- Antibody Data
- Antigen structure
- References [1]
- Comments [0]
- Validations
- Western blot [2]
- Immunocytochemistry [1]
- Immunohistochemistry [4]
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Validation data
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- Product number
- PA5-54983 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- PUS7 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant full-length protein
- Description
- Immunogen sequence: PGFDVIYPKH KIQEAYREML TADNLDIDNM RHKIRDYSLS GAYRKIIIRP QNVSWEVVAY DDPKIPLFNT DVDNLEGKTP PVFASEGKYR ALKMDFSLPP STYATMAIRE VLKMDTSIKN QTQ Highest antigen sequence identity to the following orthologs: Mouse - 95%, Rat - 95%.
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µL
- Concentration
- 0.2 mg/mL
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Submitted references Gsta4 controls apoptosis of differentiating adult oligodendrocytes during homeostasis and remyelination via the mitochondria-associated Fas-Casp8-Bid-axis.
Carlström KE, Zhu K, Ewing E, Krabbendam IE, Harris RA, Falcão AM, Jagodic M, Castelo-Branco G, Piehl F
Nature communications 2020 Aug 13;11(1):4071
Nature communications 2020 Aug 13;11(1):4071
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of PUS7 in Lane 1: Marker (kDa) 230, 130, 95, 72, 56, 36, 28, 17, 11; Lane 2: Human cell line RT-4; Lane 3: Human cell line U-251MG sp. Samples were probed using a PUS7 Polyclonal Antibody (Product # PA5-54983).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of PUS7 in Lane 1: NIH-3T3 cell lysate (Mouse embryonic fibroblast cells); Lane 2: NBT-II cell lysate (Rat Wistar bladder tumour cells). Samples were probed using a PUS7 Polyclonal Antibody (Product # PA5-54983).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent staining of PUS7 in human cell line U-251 MG shows positivity in nucleus. Samples were probed using a PUS7 Polyclonal Antibody (Product # PA5-54983).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical staining of PUS7 in human endometrium using PUS7 Polyclonal Antibody (Product # PA5-54983) shows moderate to strong nuclear positivity in glandular cells.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical staining of PUS7 in human testis using PUS7 Polyclonal Antibody (Product # PA5-54983) shows moderate to strong nuclear positivity in cells in seminiferous ducts.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical staining of PUS7 in human cervix, uterine using PUS7 Polyclonal Antibody (Product # PA5-54983) shows moderate to strong nuclear positivity in squamous epithelial cells.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical staining of PUS7 in human pancreas using PUS7 Polyclonal Antibody (Product # PA5-54983) shows moderate to strong nuclear positivity in exocrine glandular cells.