CF501816

antibody from Invitrogen Antibodies

Targeting: PUS7 FLJ20485

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Flow cytometry (Supportive data in Antibodypedia)

Antibody Data

Product number

CF501816

Provider

Invitrogen Antibodies

Product name

PUS7 Monoclonal Antibody (OTI4C6), TrueMAB™

Antibody type

Monoclonal

Antigen

Recombinant full-length protein

Reactivity

Human, Rat, Canine

Host

Mouse

Isotype

IgG

Antibody clone number

OTI4C6

Vial size

100 µg

Concentration

1 mg/mL

Storage

-20° C, Avoid Freeze/Thaw Cycles

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY PUS7 (RC203902, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 µg per lane) were separated by SDS-PAGE and immunoblotted with anti-PUS7. Positive lysates LY412749 (100 µg) and LC412749 (20 µg) can be purchased separately from OriGene.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis of extracts (35 µg) from 9 different cell lines by using anti-PUS7 monoclonal antibody.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Equivalent amounts of cell lysates (10 µg per lane) of wild-type HeLa cells (WT, Cat# LC810HELA) and PUS7-Knockout HeLa cells (KO, Cat# LC833095) were separated by SDS-PAGE and immunoblotted with anti-PUS7 monoclonal antibody TA501816(1:1000`). Then the blotted membrane was stripped and reprobed with anti-PCNA antibody as a loading control.

Immunocytochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Anti-PUS7 mouse monoclonal antibody (TA501816) Immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY PUS7(RC203902).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Anti-PUS7 mouse monoclonal antibody (TA501816) Immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY PUS7(RC203902).

Flow cytometry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Flow cytometric analysis of Jurkat cells, using anti-PUS7 antibody (TA501816), (Red), compared to a nonspecific negative control antibody (TA50011), (Blue).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Flow cytometric analysis of Hela cells, using anti-PUS7 antibody (TA501816), (Red), compared to a nonspecific negative control antibody (TA50011), (Blue).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Flow cytometric analysis of Jurkat cells, using anti-PUS7 antibody (TA501816), (Red), compared to a nonspecific negative control antibody (TA50011), (Blue).