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Western blot
ImmunoprecipitationAntibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [3]
- Immunohistochemistry [3]
- Other assay [1]
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Validation data
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- Product number
- 17707-1-AP - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- USP28 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Other
- Description
- Immunogen sequence: MTAELQQDD AAGAADGHGS SCQMLLNQLR EITGIQDPSF LHEALKASNG DITQAVSLLT DERVKEPSQD TVATEPSEVE GSAANKEVLA KVIDLTHDNK DDLQAAIALS LLESPKIQAD GRDLNRMHEA TSAETKRSKR KRCEVWGENP NPNDWRRVDG WPVGLKNVGN TCWFSAVIQS LFQLPEFRRL VLSYSLPQNV LENCRSHTEK RNIMFMQELQ YLFALMMGSN RKFVDPSAAL DLLKGAFRSS EEQQQDVSEF THKLLDWLED AFQLAVNVNS PRNKSENPMV QLFYGTFLTE GVREGKPFCN NETFGQYPLQ VNGYRNLDEC LEGAMVEGDV ELLPSDHSVK Y (1-350 aa encoded by BC065928)
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 150 µL
- Concentration
- 0.38 mg/mL
- Storage
- -20°C
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- HeLa cells were subjected to SDS PAGE followed by western blot with 17707-1-AP (USP28 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- HeLa cells were subjected to SDS PAGE followed by western blot with 17707-1-AP (USP28 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- HeLa cells were subjected to SDS PAGE followed by western blot with 17707-1-AP (USP28 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry of paraffin-embedded human breast cancer using 17707-1-AP (USP28 antibody) at dilution of 1:50 (under 10x lens).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry of paraffin-embedded human breast cancer using 17707-1-AP (USP28 antibody) at dilution of 1:50 (under 40x lens).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using (Product # 17707-1-AP) (USP28 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- IP result of anti-USP28 (IP:17707-1-AP, 4ug; Detection:17707-1-AP 1:600) with HeLa cells lysate 2800ug.
