Antibody data
- Antibody Data
- Antigen structure
- References [1]
- Comments [0]
- Validations
- Western blot [2]
- Immunohistochemistry [10]
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Validation data
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- Product number
- NBP1-82904 - Provider product page
- Provider
- Novus Biologicals
- Proper citation
- Novus Cat#NBP1-82904, RRID:AB_11003615
- Product name
- Rabbit Polyclonal USP28 Antibody
- Antibody type
- Polyclonal
- Description
- Immunogen affinity purified. Specificity of human USP28 antibody verified on a Protein Array containing target protein plus 383 other non-specific proteins.
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 0.1 ml
- Storage
- Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Submitted references Systematic validation of antibody binding and protein subcellular localization using siRNA and confocal microscopy.
Stadler C, Hjelmare M, Neumann B, Jonasson K, Pepperkok R, Uhlén M, Lundberg E
Journal of proteomics 2012 Apr 3;75(7):2236-51
Journal of proteomics 2012 Apr 3;75(7):2236-51
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: USP28 Antibody [NBP1-82904] - Analysis in mouse cell line NIH-3T3 and rat cell line NBT-II.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: USP28 Antibody [NBP1-82904] - Analysis using Anti-USP28 antibody NBP1-82904 (A) shows similar pattern to independent antibody NBP1-82905 (B).
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: USP28 Antibody [NBP1-82904] - Staining of human lymph node shows strong nuclear positivity in reaction center cells and lymphoid cells outside reaction center.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: USP28 Antibody [NBP1-82904] - Staining of human pancreas.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: USP28 Antibody [NBP1-82904] - Staining of human lymph node.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: USP28 Antibody [NBP1-82904] - Staining of human colon.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: USP28 Antibody [NBP1-82904] - Staining of human testis.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: USP28 Antibody [NBP1-82904] - Staining of human heart muscle shows strong nuclear positivity in cardiomyocytes.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: USP28 Antibody [NBP1-82904] - Staining of human rectum shows strong nuclear positivity in glandular cells.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: USP28 Antibody [NBP1-82904] - Staining of human heart muscle, lymph node, rectum and testis using Anti-USP28 antibody (A) NBP1-82904 shows similar protein distribution across tissues to independent antibody NBP1-82905 (B).
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: USP28 Antibody [NBP1-82904] - Staining of human testis shows moderate nuclear positivity in cells in seminiferous ducts.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: USP28 Antibody [NBP1-82904] - Staining of human lymph node shows strong nuclear positivity in germinal center cells.