Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [5]
- Immunohistochemistry [5]
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Validation data
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- Product number
- NBP1-83708 - Provider product page
- Provider
- Novus Biologicals
- Proper citation
- Novus Cat#NBP1-83708, RRID:AB_11017190
- Product name
- Rabbit Polyclonal SFXN1 Antibody
- Antibody type
- Polyclonal
- Description
- Immunogen affinity purified. Specificity of human, mouse, rat SFXN1 antibody verified on a Protein Array containing target protein plus 383 other non-specific proteins.
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 0.1 ml
- Storage
- Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
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Supportive validation
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- Novus Biologicals (provider)
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- Experimental details
- Western Blot: SFXN1 Antibody [NBP1-83708] - Lane 1: Marker [kDa] 230, 130, 95, 72, 56, 36, 28, 17, 11. Lane 2: Human cell line RT-4. Lane 3: Human cell line U-251MG sp
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- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: SFXN1 Antibody [NBP1-83708] - Lane 1: NIH-3T3 cell lysate (Mouse embryonic fibroblast cells). Lane 2: NBT-II cell lysate (Rat Wistar bladder tumor cells).
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- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: SFXN1 Antibody [NBP1-83708] - Analysis using Anti-SFXN1 antibody NBP1-83708 (A) shows similar pattern to independent antibody NBP2-57172 (B).
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- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: SFXN1 Antibody [NBP1-83708] - Analysis in mouse cell line NIH-3T3 and rat cell line NBT-II.
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- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: SFXN1 Antibody [NBP1-83708] - Analysis in human cell line CACO-2 and human cell line PC-3.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: SFXN1 Antibody [NBP1-83708] - Staining of human liver shows strong cytoplasmic positivity in hepatocytes.
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- Novus Biologicals (provider)
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- Experimental details
- Immunohistochemistry-Paraffin: SFXN1 Antibody [NBP1-83708] - Staining of human cerebral cortex shows strong cytoplasmic positivity in neurons.
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- Novus Biologicals (provider)
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- Experimental details
- Immunohistochemistry-Paraffin: SFXN1 Antibody [NBP1-83708] - Staining of human kidney shows strong granular cytoplasmic positivity in cells in tubules.
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- Novus Biologicals (provider)
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- Experimental details
- Immunohistochemistry-Paraffin: SFXN1 Antibody [NBP1-83708] - Staining of human liver shows very strong granular cytoplasmic positivity in hepatocytes.
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- Novus Biologicals (provider)
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- Experimental details
- Immunohistochemistry-Paraffin: SFXN1 Antibody [NBP1-83708] - Staining of human skeletal muscle shows no positivity in myocytes as expected.