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Western blot
ImmunoprecipitationAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [2]
- Immunohistochemistry [1]
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- Product number
- A300-940A - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- USP36/DUB1 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Other
- Reactivity
- Human
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µL
- Concentration
- 1 mg/mL
- Storage
- 4° C
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Detection of human USP36/DUB1 by western blot of immunoprecipitates. Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-USP36/DUB1 antibody A300-940A (lot A300-940A-2) used for IP at 6 µg per reaction. USP36/DUB1 was also immunoprecipitated by rabbit anti-USP36/DUB1 antibody A300-939A. For blotting immunoprecipitated USP36/DUB1, A300-940A was used at 1 µg/ml. Detection: Chemiluminescence with an exposure time of 10 seconds.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Detection of human USP36/DUB1 by western blot. Samples: Whole cell lysate (15 and 50 µg) from HeLa and (50 µg) 293T cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-USP36/DUB1 antibody A300-940A (lot A300-940A-2) used for WB at 0.1 µg/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Detection of human USP36/DUB1 by immunohistochemistry. Sample: FFPE section of human colon carcinoma. Antibody: Affinity purified rabbit anti- USP36/DUB1(Cat. No. A300-940A Lot 2) used at a dilution of 1:5,000 (0.2µg/ml). Detection: DAB.
