MA5-34767

antibody from Invitrogen Antibodies

Targeting: SENP1

Western blot

Immunohistochemistry

Antibody data

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Validation data

Product number: MA5-34767 - Provider product page
Provider: Invitrogen Antibodies
Product name: SENP1 Recombinant Rabbit Monoclonal Antibody (JG37-79)
Antibody type: Monoclonal
Antigen: Recombinant full-length protein
Description: Positive Control: K562, LOVO, SH-SY-5Y, SiHa, Daudi, human liver tissue, human small Intestine tissue.
Reactivity: Human, Rat
Host: Rabbit
Isotype: IgG
Antibody clone number: JG37-79
Vial size: 100 µL
Concentration: 1 mg/mL
Storage: -20° C, Avoid Freeze/Thaw Cycles, store in dark

SENP1 protein structure - MA5-34767 shown in red.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Immunofluorescence analysis of sentrin/SUMO-specific protease SENP1 was performed using 70% confluent log phase A549 cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 15 minutes, and blocked with 2% BSA for 1 hour at room temperature. The cells were labeled with SENP1 Recombinant Rabbit Monoclonal Antibody (JG37-79) (Product # MA5-34767) at 1:100 dilution in 0.1% BSA, incubated at 4 degree celsius overnight and then labeled with Donkey anti-Rabbit IgG (H+L) Highly Cross-Adsorbed Secondary Antibody, Alexa Fluor Plus 488 (Product # A32790, 1:2000 dilution), for 45 minutes at room temperature (Panel a: Green). Nuclei (Panel b:Blue) were stained with ProLong™ Diamond Antifade Mountant with DAPI (Product # P36962). F-actin (Panel c: Red) was stained with Rhodamine Phalloidin (Product # R415, 1:300 dilution). Panel d represents the merged image showing nucleus and cytoplasmic localization. Panel e represents control cells with no primary antibody to assess background. The images were captured at 60X with oil immersion magnification.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Immunofluorescent analysis of SENP1 in SiHa cells (green). Samples were fixed in paraformaldehyde and permeabilised with 0.25% Triton X100/PBS, incubated with SENP1 monoclonal antibody (Product # MA5-34767), followed by DAPI (blue).

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Immunofluorescent analysis of SENP1 in SH-SY-5Y cells (green). Samples were fixed in paraformaldehyde and permeabilised with 0.25% Triton X100/PBS, incubated with SENP1 monoclonal antibody (Product # MA5-34767), followed by DAPI (blue).

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Immunofluorescent analysis of SENP1 in LOVO cells (green). Samples were fixed in paraformaldehyde and permeabilised with 0.25% Triton X100/PBS, incubated with SENP1 monoclonal antibody (Product # MA5-34767), followed by DAPI (blue).