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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [3]
- Comments [0]
- Validations
- Western blot [1]
- Immunohistochemistry [1]
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- Product number
- PAB4318 - Provider product page
- Provider
- Abnova Corporation
- Proper citation
- Abnova Corporation Cat#PAB4318, RRID:AB_1582040
- Product name
- ZMPSTE24 polyclonal antibody
- Antibody type
- Polyclonal
- Description
- Rabbit polyclonal antibody raised against synthetic peptide of ZMPSTE24.
- Storage
- Store at 4°C. For long term storage store at -20°C.Aliquot to avoid repeated freezing and thawing.
Submitted references Identification and chromosomal location of two human genes encoding enzymes potentially involved in proteolytic maturation of farnesylated proteins.
Identification of a human cDNA encoding a novel protein structurally related to the yeast membrane-associated metalloprotease, Ste24p.
Dual roles for Ste24p in yeast a-factor maturation: NH2-terminal proteolysis and COOH-terminal CAAX processing.
Freije JM, Blay P, Pendás AM, Cadiñanos J, Crespo P, López-Otín C
Genomics 1999 Jun 15;58(3):270-80
Genomics 1999 Jun 15;58(3):270-80
Identification of a human cDNA encoding a novel protein structurally related to the yeast membrane-associated metalloprotease, Ste24p.
Kumagai H, Kawamura Y, Yanagisawa K, Komano H
Biochimica et biophysica acta 1999 Feb 2;1426(3):468-74
Biochimica et biophysica acta 1999 Feb 2;1426(3):468-74
Dual roles for Ste24p in yeast a-factor maturation: NH2-terminal proteolysis and COOH-terminal CAAX processing.
Tam A, Nouvet FJ, Fujimura-Kamada K, Slunt H, Sisodia SS, Michaelis S
The Journal of cell biology 1998 Aug 10;142(3):635-49
The Journal of cell biology 1998 Aug 10;142(3):635-49
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Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- The ZMPSTE24 polyclonal antibody (Cat # PAB4318) is used in Western blot to detect ZMPSTE24 in T-47D cell lysate .
Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Formalin-fixed and paraffin-embedded human hepatocellular carcinoma tissue reacted with ZMPSTE24 polyclonal antibody (Cat # PAB4318) , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining . This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated . HC = hepatocarcinoma .
- Validation comment
- Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
