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- Antibody Data
- Antigen structure
- References [1]
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- Validations
- Western blot [2]
- Other assay [1]
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- Product number
- PA1-16965 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- ZMPSTE24 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Synthetic peptide
- Description
- Suggested positive control: human testis protein.
- Reactivity
- Human
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µL
- Concentration
- 1 mg/mL
- Storage
- -20° C, Avoid Freeze/Thaw Cycles
Submitted references ZMPSTE24 Regulates SARS-CoV-2 Spike Protein-enhanced Expression of Endothelial PAI-1.
Han M, Pandey D
American journal of respiratory cell and molecular biology 2021 Sep;65(3):300-308
American journal of respiratory cell and molecular biology 2021 Sep;65(3):300-308
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot detection of ZMPSTE24 in human testis using Product # PA1-16965.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of ZMPSTE24 in human testis. Sample was incubated in ZMPSTE24 polyclonal antibody (Product # PA1-16965).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Figure 4. ZMPSTE24 (zinc metallopeptidase STE24) is critical for ACE2 cellular expression. ( A ) Normal (WT) and ZMPSTE24-knockout (ZMP-KO) HeLa cells were transfected with or without ACE2 (C-terminal FLAG-tagged) plasmid and treated with or without rSARS-CoV-2-S1 protein. Cell lysates were immunoblotted with anti-ACE2, anti-FLAG, and anti-GAPDH antibodies. * P < 0.05 versus ACE2-expressing WT HeLa cells ( n = 3). ( B ) Conditioned media from WT or ZMP-KO HeLa cells transfected with FLAG-ACE2 were immunoblotted with ectodomain-specific anti-ACE2 antibody and stained with ponceau to determine total protein loading. * P < 0.05 versus ACE2-expressing WT HeLa cells ( n = 3). ( C ) Schematic of ACE2 depicts the N-terminal ectodomain, TM domain, and Cyto domain. Leucine584 is a potential ZMPSTE24 cleavage site (RPLLNYF). ( D ) WT and ZMP-KO HeLa cells were transduced with adenoviruses encoding ACE2, and cell lysates were immunoblotted with ectodomain-specific anti-ACE2 and anti-GAPDH antibodies ( n = 3). ( E ) WT HeLa cells cotransfected with either ACE2 and GFP (control) or ACE2 and GFP-tagged progerin were immunoblotted with ectodomain-specific anti-ACE2, anti-GFP, and anti-GAPDH antibodies ( n = 3). Cyto = cytoplasmic; ns = not statistically significant; TM = transmembrane.
