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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [3]
- Immunohistochemistry [1]
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- Product number
- CF809367 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- THOC3 Monoclonal Antibody (OTI4H6), TrueMAB™
- Antibody type
- Monoclonal
- Antigen
- Recombinant protein fragment
- Reactivity
- Human, Mouse, Rat
- Host
- Mouse
- Isotype
- IgG
- Antibody clone number
- OTI4H6
- Vial size
- 100 µg
- Concentration
- 1 mg/mL
- Storage
- -20° C, Avoid Freeze/Thaw Cycles
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY THOC3 (RC201698, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 µg per lane) were separated by SDS-PAGE and immunoblotted with anti-THOC3. Positive lysates LY403157 (100 µg) and LC403157 (20 µg) can be purchased separately from OriGene.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Equivalent amounts of cell lysates (10 µg per lane) of wild-type HeLa cells (WT, Cat# LC810HELA) and THOC3-Knockout HeLa cells (KO, Cat# LC833215) were separated by SDS-PAGE and immunoblotted with anti-THOC3 monoclonal antibody TA809367(1:200`). Then the blotted membrane was stripped and reprobed with anti-PCNA antibody as a loading control.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of extracts (35 µg) from 3 different cell lines by using anti-THOC3 monoclonal antibody. (1:500)
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical staining of paraffin-embedded human lung tissue within the normal limits using anti-THOC3 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3 min, TA809367)(1:500).
