MA5-34645

antibody from Invitrogen Antibodies

Targeting: PSME1 IFI5111, PA28alpha

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunoprecipitation (Recommended by provider)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

MA5-34645

Provider

Invitrogen Antibodies

Product name

PSME1 Recombinant Rabbit Monoclonal Antibody (JU60-33)

Antibody type

Monoclonal

Antigen

Recombinant full-length protein

Description

Positive Control: MCF-7, rat lung tissue lysate, HepG2, MCF-7, PC-3M, rat kidney tissue, human tonsil tissue, human liver cancer tissue, human prostate tissue, mouse small intestine tissue.

Reactivity

Human

Host

Rabbit

Isotype

IgG

Antibody clone number

JU60-33

Vial size

100 µL

Concentration

1 mg/mL

Storage

-20° C, Avoid Freeze/Thaw Cycles, store in dark

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis of PSME1 in MCF-7 cell (1) and rat lung tissue (2) lysate. Samples were incubated with PSME1 monoclonal antibody (Product # MA5-34645), at a dilution of 1:500.

Immunocytochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescent analysis of PSME1 in PC-3M cells (green). Samples were fixed in paraformaldehyde and permeabilised with 0.25% Triton X100/PBS, incubated with PSME1 monoclonal antibody (Product # MA5-34645), followed by DAPI (blue).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescent analysis of PSME1 in MCF-7 cells (green). Samples were fixed in paraformaldehyde and permeabilised with 0.25% Triton X100/PBS, incubated with PSME1 monoclonal antibody (Product # MA5-34645), followed by DAPI (blue).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescent analysis of PSME1 in HepG2 cells (green). Samples were fixed in paraformaldehyde and permeabilised with 0.25% Triton X100/PBS, incubated with PSME1 monoclonal antibody (Product # MA5-34645), followed by DAPI (blue).

Immunohistochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry analysis of PSME1 in paraffin-embedded rat kidney tissue. Samples were incubated with PSME1 monoclonal antibody (Product # MA5-34645), and followed by hematoxylin.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry analysis of PSME1 in paraffin-embedded mouse small intestine tissue. Samples were incubated with PSME1 monoclonal antibody (Product # MA5-34645), and followed by hematoxylin.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry analysis of PSME1 in paraffin-embedded human tonsil tissue. Samples were incubated with PSME1 monoclonal antibody (Product # MA5-34645), and followed by hematoxylin.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry analysis of PSME1 in paraffin-embedded human prostate tissue. Samples were incubated with PSME1 monoclonal antibody (Product # MA5-34645), and followed by hematoxylin.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry analysis of PSME1 in paraffin-embedded human liver cancer tissue. Samples were incubated with PSME1 monoclonal antibody (Product # MA5-34645), and followed by hematoxylin.