PA1-778

antibody from Invitrogen Antibodies

Targeting: TPH2 FLJ37295, NTPH

Provider product page for PA1-778

Western blot

Immunoprecipitation

Other assay

Antibody data

Antibody Data
Antigen structure
References [13]
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Validations
Other assay [13]

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Product number: PA1-778 - Provider product page
Provider: Invitrogen Antibodies
Product name: TPH2 Polyclonal Antibody
Antibody type: Polyclonal
Antigen: Synthetic peptide
Description: PA1-778 detects TPH2 in human, mouse, rat, rabbit and non-human primate samples. PA1-778 has been successfully used in immunocytochemistry, immunoprecipitation, and Western blot procedures. By Western blot PA1-778 detects a 56 kDa protein representing TPH2. The PA1-778 immunogen is a synthetic peptide corresponding to the N-terminal region of TPH2.
Reactivity: Human, Mouse, Rat, Rabbit
Host: Rabbit
Isotype: IgG
Vial size: 100 µL
Concentration: 0.5 mg/mL
Storage: 4° C

TPH2 protein structure - PA1-778 shown in red.

Supportive validation

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Experimental details: NULL

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
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Experimental details: NULL

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
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Experimental details: NULL

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
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Experimental details: NULL

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
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Experimental details: NULL

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
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Experimental details: Immunoprecipitation of TPH2 from raphe nuclei isolated from Sprague-Dawley rat brains using TPH2 Polyclonal Antibody (Product # PA1-778). Product # PA1-778 was coupled to magnetic beads and added to raphe nuclei whole cell extracts for 15 minutes at RT. Immunoprecipitated proteins were assessed by Western blotting (WB), using either mouse anti-TPH2 (Ms TPH2, Lane 2) or sheep anti-TPH2 antibodies (Sh TPH2, Lane 3). As a positive control for the Western blot, non-immunoprecipitated brain lysates were probed with the mouse anti-TPH2 antibody (Lane 1). As a specificity control, a blot containing immunoprecipitated proteins was probed with an antibody specific for Tyrosine Hydroxylase (TH, Lane 4). Data courtesy of the Innovators Program.

Supportive validation

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Experimental details: Figure 1 Polymerase chain reaction and immunocytochemistry results. (A) Expression of the Cre (left) and TPH2 (right) marker genes. (B) Immunofluorescence in 5-HT-positive neurons in the wild-type group (magnification, x20). (C) 5-HT-positive neurons were lost in the TPH2-knockout group (magnification, x20). Scale bar=20 mum. Mlf, medial longitudinal fasciculus; 5-HT, 5-hydroxytryptamine; TPH2, tryptophan hydroxylase 2.

Supportive validation

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Experimental details: FIGURE 1 Generation of conditional knockout mice, reporter expression and alteration of 5-HT and its metabolite in the transgenic mice. (A) An experimental scheme representing the time of injection, reporter assay, HPLC measurements and the duration when different behavioral battery tests were performed followed by molecular analysis. (B) Confirmation of Cre-recombination in the tDtomato reporter mice using immunohistochemistry with Tph2 antibody in tamoxifen injected and corn oil injected animals ( N = 4). (C) Western blotting with TrkB monoclonal antibody on the mid-brain samples from the control and TG mice ( N = 4). (D) (a) The total levels of 5-HT was measured by HPLC in naive animal''s different brain regions such as the PFC, HC, and MB. The levels in TG mice were significantly upregulated in the PFC and MB. (b) The levels of 5HIAA did not change in these brain regions. (c) The 5HIAA/5-HT ratio was also measured in these regions. The ratio was significantly down regulated in the MB of the TG mice. (d) The levels of DA were significantly upregulated in the PFC and MB of the TG mice ( N = 4). (E) q-RT PCR results of the transcripts Tph2 in the MB and Bdnf in the PFC was increased. The transcript for 5ht2b was reduced in the PFC of the TG mice. No change in Bdnf and 5ht2b transcripts in the HC was observed ( N = 4). (F) In situ hybridization with Bdnf probe in the PFC of Ctrl and TG mice shows a robust expression ( N = 3). Scale bar represents 100 mum, Student''s t -test,

Supportive validation

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Experimental details: 6 Figure Examination of sex differences in monoaminergic descending pathways from the brain to the spinal cord Representative micrographs of immunofluorescence staining of tyrosine hydroxylase (TH: red, left panels), Fluorogold (FG: green, middle panels) and merged images (right panels) in the A11 region ( A ) and tryptophan hydroxylase 2 (TPH2: red, left panels), Fluorogold (FG: green, middle panels) and merged images (right panels) in the medullary raphe nuclei ( B ) of male rats (upper panels) and female rats (lower panels). Bar graphs summarize the number of TH-positive or TPH2-positive (left panel), FG-positive (middle panel) and double-positive (right panel) cells in male and female rats. Scale bar: 200 mum. * P < 0.05, significant difference between the groups.

Supportive validation

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Experimental details: Fig. 8 Effects of MDMA doses and environments, respectively, on initiating and intensifying serotonergic injury. Serotonergic axonal retrograde transportation was used to estimate those effects. a Timeline of MDMA or SAL treatment followed by T cor measurement (day 1), fluorogold administration (FG; day 7) and tissue sectioning (day 14). b Immunohistochemical staining of tryptophan hydroxylase-2 (TpH2)-containing neurons in the DRN retrograded with FG. Photomicrographs show representative changes in FG-positive (FG + ) or/and TpH2-positive (TpH2 + ) neurons in the DRN. The top row, the FG + neurons; the middle row, the TpH2 + neurons; the bottom row, FG + /TpH2 + . c Cell counts of FG + and FG + /TpH2 + neurons ( N = 4-6). ** P < 0.01 vs. the SAL control, examined using unpaired t-test. CTL-S , saline as control; HD-H - , high-dose-induced hypothermia; HD-H + , high-dose-induced hyperthermia; LD-H + , low-dose-induced hyperthermia

Supportive validation

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Experimental details: 3 FIGURE TPH2 protein expression in wild-type and Galphaq knockout mice. Representative western blots for protein quantitation in male and female synaptosomes from wild-type and Galphaq knockout mice from midbrain (Mid, a), frontal cortex (FC, c) and hippocampus (HC, e). Proteins were detected as follows: TPH2 (Thermo Fisher, rabbit, green) and actin (Sigma, mouse, red). Protein quantitation for each brain region, midbrain (b), frontal cortex (d) and hippocampus (f). Data are presented as intensity values normalised against actin and relative to the reference sample (ref) +- SEM , n = 7-10. Midbrain, no significant (ns) differences; frontal cortex, sex: F (1,15) = 17.37, p = .0008; hippocampus, no significant (ns) differences. Statistical analysis by two-way ANOVA, pairwise comparison Tukey post-test corrected for multiple comparison, * p < .05

Supportive validation

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Experimental details: Fig. 5. Representative immunohistochemistry for tryptophan hydroxylase 2 (TPH2) in the dorsal raphe nuclei (DRN) and median raphe nuclei (MRN) in all groups. The positive intensities of TPH2 in the DRN and MRN were increased in the DIN-500 and DIN-0+CUMS groups, as compared to those in the DIN-0 group. The increase in the DIN-0+CUMS group was counteracted in the DIN-500+CUMS and DIN-2500+CUMS groups. CUMS, chronic unpredictable mild stress.

Supportive validation

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Experimental details: Figure 5 Effects of BPA on protein levels of tryptophan hydroxylase type 2 (Tph2) in prefrontal cortex of BPA-treated male and female rats and their respective controls. * at least p