Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [3]
- Immunohistochemistry [1]
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Validation data
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- Product number
- LS-C344720 - Provider product page
- Provider
- LSBio
- Product name
- NAMPT / Visfatin Antibody (clone OMNI379, Biotin) LS-C344720
- Antibody type
- Monoclonal
- Description
- Protein G purified from tissue culture supernatant
- Reactivity
- Human, Mouse, Rat
- Host
- Mouse
- Conjugate
- Biotin
- Isotype
- IgG
- Antibody clone number
- OMNI379
- Storage
- Short term: store at 4°C. Long term: store at -20°C. Avoid freeze-thaw cycles.
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Supportive validation
- Submitted by
- LSBio (provider)
- Enhanced method
- Genetic validation
- Main image
- Experimental details
- Western blot analysis of 3T3-L1 cell lysate using anti-Nampt (Visfatin/PBEF) mAb (OMNI379) at 1: 2,000 dilution. 1. IBMX. + DEX. - treated 3T3-L1 cell lysate. 2. IBMX. + DEX. + Insulin - treated 3T3-L1 cell lysate. This image was taken for the unconjugated form of this product. Other forms have not been tested.
- Submitted by
- LSBio (provider)
- Enhanced method
- Genetic validation
- Main image
- Experimental details
- Western blot analysis using anti-Nampt (Visfatin-PBEF), mAb (OMNI379) at 1: 2,000 dilution. 1. Human Nampt(Visfatin/PBEF) (His-tagged). 2. Human Nampt(Visfatin/PBEF) (FLAG-tagged). 3. Mouse Nampt(Visfatin/PBEF) (His-tagged). 4. Rat Nampt(Visfatin/PBEF) (His-tagged). 5. Human Nampt(Visfatin/PBEF) (His-tagged). 6. LPS-treated human peripheral blood leukocyte lysate. This image was taken for the unconjugated form of this product. Other forms have not been tested.
- Submitted by
- LSBio (provider)
- Enhanced method
- Genetic validation
- Main image
- Experimental details
- Immunoprecipitation of recombinant human Nampt(Visfatin/PBEF) proteins using anti-Nampt (Visfatin-PBEF), mAb (OMNI379). Recombinant human Nampt(Visfatin/PBEF) proteins at different concentrations were precipitated. The precipitated proteins were separated by SDS-PAGE, electroblotted, and visualized by western blot with anti-human Nampt(Visfatin/PBEF) pAb. This image was taken for the unconjugated form of this product. Other forms have not been tested.
Supportive validation
- Submitted by
- LSBio (provider)
- Enhanced method
- Genetic validation
- Main image
- Experimental details
- Immunohistochemical staining of Nampt using anti-Nampt (OMNI379) (Biotin) in human tissue (1:500 dilution). Immunoperoxidase staining of formalin-fixed, paraffin-embedded human kidney showing cytoplasmic staining (200x, brown color). This image was taken for the unconjugated form of this product. Other forms have not been tested.