LS-C348735

antibody from LSBio

Targeting: NAMPT PBEF, PBEF1

Western blot (Supportive data in Antibodypedia)

ELISA (Recommended by provider)

Immunocytochemistry (Data presented on provider website)

Immunoprecipitation (Recommended by provider)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

LS-C348735

Provider

LSBio

Product name

Anti-NAMPT / Visfatin Antibody (clone 14A5) LS-C348735

Antibody type

Monoclonal

Description

Protein A purified

Reactivity

Human, Mouse

Host

Rat

Isotype

IgG

Antibody clone number

14A5

Vial size

100µg

Storage

Short term 4°C, long term aliquot and store at -20°C, avoid freeze thaw cycles. Store undiluted.

Western blot

Supportive validation

Submitted by

LSBio (provider)

Main image

Experimental details

NAMPT is expressed in the cytoplasm. Equal amouns of cytosolic and membrane proteins from anti-CD3 stimulated spleen cells were separated by SDS-PAGE and blotted with 14A5 mAb (0. 1ug/ml). Secondary Ab: anti-Rat-HPRT:

Supportive validation

Submitted by

LSBio (provider)

Main image

Experimental details

Cell extract from fetal mouse skin dendritic cell line (FSDC) was separated by SDS-PAGE and blotted with 14A5 mAb (0.1 ug/ml). Secondary Ab: anti Rat-HPRT

Immunohistochemistry

Supportive validation

Submitted by

LSBio (provider)

Main image

Experimental details

Immunostaining of Mouse spleen frozen section (14A5: 10 ug /ml). Secondary Ab: Biotinylated anti-Rat