Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [2]
- Immunohistochemistry [1]
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Validation data
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- Product number
- LS-C348735 - Provider product page
- Provider
- LSBio
- Product name
- Anti-NAMPT / Visfatin Antibody (clone 14A5) LS-C348735
- Antibody type
- Monoclonal
- Description
- Protein A purified
- Reactivity
- Human, Mouse
- Host
- Rat
- Isotype
- IgG
- Antibody clone number
- 14A5
- Vial size
- 100µg
- Storage
- Short term 4°C, long term aliquot and store at -20°C, avoid freeze thaw cycles. Store undiluted.
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Supportive validation
- Submitted by
- LSBio (provider)
- Main image
- Experimental details
- NAMPT is expressed in the cytoplasm. Equal amouns of cytosolic and membrane proteins from anti-CD3 stimulated spleen cells were separated by SDS-PAGE and blotted with 14A5 mAb (0. 1ug/ml). Secondary Ab: anti-Rat-HPRT:
- Submitted by
- LSBio (provider)
- Main image
- Experimental details
- Cell extract from fetal mouse skin dendritic cell line (FSDC) was separated by SDS-PAGE and blotted with 14A5 mAb (0.1 ug/ml). Secondary Ab: anti Rat-HPRT
Supportive validation
- Submitted by
- LSBio (provider)
- Main image
- Experimental details
- Immunostaining of Mouse spleen frozen section (14A5: 10 ug /ml). Secondary Ab: Biotinylated anti-Rat