LS-B12879

antibody from LSBio

Targeting: NAMPT PBEF, PBEF1

Western blot (Supportive data in Antibodypedia)

ELISA (Recommended by provider)

Immunocytochemistry (Data presented on provider website)

Immunoprecipitation (Recommended by provider)

Immunohistochemistry (Data presented on provider website)

Antibody Data

Product number

LS-B12879

Provider

LSBio

Product name

IHC-plus™ NAMPT / Visfatin Antibody (clone OMNI379) LS-B12879

Antibody type

Monoclonal

Description

Protein G purified from tissue culture supernatant

Reactivity

Human, Mouse, Rat

Host

Mouse

Isotype

IgG

Antibody clone number

OMNI379

Storage

Short term: store at 4°C. Long term: store at -20°C. Avoid freeze-thaw cycles.

Western blot

Supportive validation

Submitted by

LSBio (provider)

Main image

Experimental details

Western blot analysis of 3T3-L1 cell lysate using anti-Nampt (Visfatin/PBEF) mAb (OMNI379) at 1: 2,000 dilution. 1. IBMX. + DEX. - treated 3T3-L1 cell lysate. 2. IBMX. + DEX. + Insulin - treated 3T3-L1 cell lysate.

Supportive validation

Submitted by

LSBio (provider)

Main image

Experimental details

Western blot analysis using anti-Nampt (Visfatin-PBEF), mAb (OMNI379) at 1: 2,000 dilution. 1. Human Nampt(Visfatin/PBEF) (His-tagged). 2. Human Nampt(Visfatin/PBEF) (FLAG-tagged). 3. Mouse Nampt(Visfatin/PBEF) (His-tagged). 4. Rat Nampt(Visfatin/PBEF) (His-tagged). 5. Human Nampt(Visfatin/PBEF) (His-tagged). 6. LPS-treated human peripheral blood leukocyte lysate.

Supportive validation

Submitted by

LSBio (provider)

Main image

Experimental details

Immunoprecipitation of recombinant human Nampt (Visfatin/PBEF) proteins using anti-Nampt (Visfatin-PBEF), mAb (OMNI379). Recombinant human Nampt (Visfatin/PBEF) proteins at different concentrations were precipitated. The precipitated proteins were separated by SDS-PAGE, electroblotted, and visualized by western blot with anti-human Nampt(Visfatin/PBEF) pAb.