LS-C376222

antibody from LSBio

Targeting: NAMPT PBEF, PBEF1

ELISA (Recommended by provider)

Immunoprecipitation (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

LS-C376222

Provider

LSBio

Product name

NAMPT / Visfatin Antibody (aa1-280) LS-C376222

Antibody type

Polyclonal

Description

Immunoaffinity purified

Reactivity

Human

Host

Rabbit

Isotype

IgG

Storage

Short term: -20°C; Long term: -80°C; Avoid freeze-thaw cycles.

Immunoprecipitation

Supportive validation

Submitted by

LSBio (provider)

Enhanced method

Genetic validation

Main image

Experimental details

Immunoprecipitating NAMPT in MCF-7 whole cell lysate Lane 1: Rabbit control IgG instead of NAMPT Antibody in MCF-7 whole cell lysate.For western blotting, a HRP-conjugated Protein G antibody was used as the secondary antibody (1/2000) Lane 2: NAMPT Antibody (8µg) + MCF-7 whole cell lysate (500µg) Lane 3: MCF-7 whole cell lysate (10µg)

Supportive validation

Submitted by

LSBio (provider)

Main image

Experimental details

Immunoprecipitating NAMPT in MCF-7 whole cell lysate Lane 1: Rabbit control IgG instead of NAMPT Antibody in MCF-7 whole cell lysate.For western blotting, a HRP-conjugated Protein G antibody was used as the secondary antibody (1/2000) Lane 2: NAMPT Antibody (8µg) + MCF-7 whole cell lysate (500µg) Lane 3: MCF-7 whole cell lysate (10µg)

Supportive validation

Submitted by

LSBio (provider)

Main image

Experimental details

Immunoprecipitating NAMPT in MCF-7 whole cell lysate Lane 1: Rabbit control IgG instead of NAMPT Antibody in MCF-7 whole cell lysate.For western blotting, a HRP-conjugated Protein G antibody was used as the secondary antibody (1/2000) Lane 2: NAMPT Antibody (8µg) + MCF-7 whole cell lysate (500µg) Lane 3: MCF-7 whole cell lysate (10µg)

Immunohistochemistry

Supportive validation

Submitted by

LSBio (provider)

Enhanced method

Genetic validation

Main image

Experimental details

Immunohistochemistry of paraffin-embedded human liver using antibody at 1:100 dilution.

Supportive validation

Submitted by

LSBio (provider)

Enhanced method

Genetic validation

Main image

Experimental details

Immunohistochemistry of paraffin-embedded human kidney using antibody at 1:100 dilution.