Antibody data
- Antibody Data
- Antigen structure
- References [8]
- Comments [0]
- Validations
- Western blot [5]
- Immunohistochemistry [1]
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Validation data
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- Product number
- NB100-594 - Provider product page
- Provider
- Novus Biologicals
- Proper citation
- Novus Cat#NB100-594, RRID:AB_10002682
- Product name
- Rabbit Polyclonal PBEF/Visfatin/NAMPT Antibody
- Antibody type
- Polyclonal
- Description
- Immunogen affinity purified.
- Reactivity
- Human, Mouse
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 ul
- Concentration
- 1.0 mg/ml
- Storage
- Store at 4C. Do not freeze.
Submitted references Visfatin/pre-B-cell colony-enhancing factor (PBEF), a proinflammatory and cell motility-changing factor in rheumatoid arthritis.
A combination of the metabolic enzyme inhibitor APO866 and the immune adjuvant L-1-methyl tryptophan induces additive antitumor activity.
Skeletal muscle NAMPT is induced by exercise in humans.
Muscle contractile activity regulates Sirt3 protein expression in rat skeletal muscles.
Regulation of inflammatory cytokine expression in pulmonary epithelial cells by pre-B-cell colony-enhancing factor via a nonenzymatic and AP-1-dependent mechanism.
Extension of human cell lifespan by nicotinamide phosphoribosyltransferase.
Increased visfatin messenger ribonucleic acid and protein levels in adipose tissue and adipocytes in women with polycystic ovary syndrome: parallel increase in plasma visfatin.
Pre-B-cell colony-enhancing factor as a potential novel biomarker in acute lung injury.
Meier FM, Frommer KW, Peters MA, Brentano F, Lefèvre S, Schröder D, Kyburz D, Steinmeyer J, Rehart S, Gay S, Müller-Ladner U, Neumann E
The Journal of biological chemistry 2012 Aug 17;287(34):28378-85
The Journal of biological chemistry 2012 Aug 17;287(34):28378-85
A combination of the metabolic enzyme inhibitor APO866 and the immune adjuvant L-1-methyl tryptophan induces additive antitumor activity.
Yang HJ, Yen MC, Lin CC, Lin CM, Chen YL, Weng TY, Huang TT, Wu CL, Lai MD
Experimental biology and medicine (Maywood, N.J.) 2010 Jul;235(7):869-76
Experimental biology and medicine (Maywood, N.J.) 2010 Jul;235(7):869-76
Skeletal muscle NAMPT is induced by exercise in humans.
Costford SR, Bajpeyi S, Pasarica M, Albarado DC, Thomas SC, Xie H, Church TS, Jubrias SA, Conley KE, Smith SR
American journal of physiology. Endocrinology and metabolism 2010 Jan;298(1):E117-26
American journal of physiology. Endocrinology and metabolism 2010 Jan;298(1):E117-26
Muscle contractile activity regulates Sirt3 protein expression in rat skeletal muscles.
Hokari F, Kawasaki E, Sakai A, Koshinaka K, Sakuma K, Kawanaka K
Journal of applied physiology (Bethesda, Md. : 1985) 2010 Aug;109(2):332-40
Journal of applied physiology (Bethesda, Md. : 1985) 2010 Aug;109(2):332-40
Regulation of inflammatory cytokine expression in pulmonary epithelial cells by pre-B-cell colony-enhancing factor via a nonenzymatic and AP-1-dependent mechanism.
Liu P, Li H, Cepeda J, Xia Y, Kempf JA, Ye H, Zhang LQ, Ye SQ
The Journal of biological chemistry 2009 Oct 2;284(40):27344-51
The Journal of biological chemistry 2009 Oct 2;284(40):27344-51
Extension of human cell lifespan by nicotinamide phosphoribosyltransferase.
van der Veer E, Ho C, O'Neil C, Barbosa N, Scott R, Cregan SP, Pickering JG
The Journal of biological chemistry 2007 Apr 13;282(15):10841-5
The Journal of biological chemistry 2007 Apr 13;282(15):10841-5
Increased visfatin messenger ribonucleic acid and protein levels in adipose tissue and adipocytes in women with polycystic ovary syndrome: parallel increase in plasma visfatin.
Tan BK, Chen J, Digby JE, Keay SD, Kennedy CR, Randeva HS
The Journal of clinical endocrinology and metabolism 2006 Dec;91(12):5022-8
The Journal of clinical endocrinology and metabolism 2006 Dec;91(12):5022-8
Pre-B-cell colony-enhancing factor as a potential novel biomarker in acute lung injury.
Ye SQ, Simon BA, Maloney JP, Zambelli-Weiner A, Gao L, Grant A, Easley RB, McVerry BJ, Tuder RM, Standiford T, Brower RG, Barnes KC, Garcia JG
American journal of respiratory and critical care medicine 2005 Feb 15;171(4):361-70
American journal of respiratory and critical care medicine 2005 Feb 15;171(4):361-70
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: PBEF/Visfatin/NAMPT Antibody [NB100-594] - Whole cell lysate from 293T, HeLa, Jurkat, mouse TCMK-1, and mouse NIH3T3 cells.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: PBEF/Visfatin/NAMPT Antibody [NB100-594] - Whole cell lysate from 293T cells.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: PBEF/Visfatin/NAMPT Antibody [NB100-594] - Detection of Human and Mouse PBEF by Western Blot. Samples: Whole cell lysate (50 ug) from HeLa, 293T, and mouse NIH3T3 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-PBEF antibody NB100-594 used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 10 seconds.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: PBEF/Visfatin/NAMPT Antibody [NB100-594] - Samples: Whole cell lysate (50 ug) from HeLa, RKO, MCF-7, TCMK-1, and NIH 3T3 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-PBEF antibody used for WB at 0.04 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: PBEF/Visfatin/NAMPT Antibody [NB100-594] - Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-PBEF antibody used for IP at 6 ug per reaction. PBEF was also immunoprecipitated by a previous lot of this antibody and rabbit anti-Visfatin antibody. For blotting immunoprecipitated PBEF, Antibody was used at 0.04 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry: PBEF/Visfatin/NAMPT Antibody [NB100-594] - Sample: FFPE section of human placental fetal membrane. Antibody: Affinity purified rabbit anti-PBEF used at a dilution of 1:5,000 (0.2ug/ml). Detection: DAB