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Western blot
ELISAAntibody data
- Antibody Data
- Antigen structure
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- Western blot [1]
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- Product number
- PB10009 - Provider product page
- Provider
- Boster Biological Technology
- Product name
- Anti-Visfatin/NAMPT Antibody Picoband™
- Antibody type
- Polyclonal
- Description
- Polyclonal antibody for PBEF/NAMPT detection. Host: Rabbit.Size: 100μg/vial. Tested applications: ELISA. Reactive species: Human. PBEF/NAMPT information: Molecular Weight: 55521 MW; Subcellular Localization: Nucleus . Cytoplasm . Secreted . Under non-inflammatory conditions, visfatin predominantly exhibits a granular pattern within the nucleus. Secreted by endothelial cells upon IL-1beta stimulation. Abundantly secreted in milk, reaching 100-fold higher concentrations compared to maternal serum; Tissue Specificity: Expressed in large amounts in bone marrow, liver tissue, and muscle. Also present in heart, placenta, lung, and kidney tissues.
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Vial size
- 100μg/vial
- Concentration
- Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
- Storage
- At -20°C for one year. After reconstitution, at 4°C for one month. It can also be aliquoted and stored frozen at -20°C for a longer time. Avoid repeated freezing and thawing.
- Handling
- Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
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Supportive validation
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- Boster Biological Technology (provider)
- Main image
- Experimental details
- Western blot analysis of Visfatin expression in rat brain extract (lane 1), mouse cardiac muscle extract (lane 2), and human placenta extract (lane 3). Visfatin at 56KD was detected using rabbit anti-Visfatin Antigen Affinity purified polyclonal antibody (Catalog # PB10009) at 0.5 μg/mL. The blot was developed using chemiluminescence (ECL) method (Catalog # EK1002).
- Additional image
