710115

antibody from Invitrogen Antibodies

Targeting: NAMPT PBEF, PBEF1

Western blot (Supportive data in Antibodypedia)

Antibody Data

Product number

710115

Provider

Invitrogen Antibodies

Product name

NAMPT Recombinant Polyclonal Antibody (21HCLC)

Antibody type

Polyclonal

Antigen

Recombinant full-length protein

Reactivity

Human

Host

Rabbit

Isotype

IgG

Antibody clone number

21HCLC

Vial size

100 µg

Concentration

0.5 mg/mL

Storage

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis of Visfatin was performed by loading 20 µg of Rat Brain lysate using Novex®NuPAGE®4-12% Bis-Tris gel (Product # NP0321BOX), XCell SureLock Electrophoresis System (Product # EI0002), Novex® Sharp Pre-Stained Protein Standard (Product # LC5800), and iBlot® Dry Blotting System (Product # IB21001). Proteins were transferred to a nitrocellulose membrane and blocked with 5% skim milk for 1 hour at room temperature. Visfatin was detected at ~55 kDa using Visfatin Recombinant Rabbit Polyclonal Antibody (Product # 710115) at 1-2 µg/mL in 2.5% skim milk at 4°C overnight on a rocking platform. Goat anti-Rabbit IgG - HRP Secondary Antibody (Product # G-21234) at 1:5000 dilution was used and chemiluminescent detection was performed using Pierce™ ECL Western blotting Substrate (Product # 32106).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis of Visfatin in whole cell extracts of K562 using a Visfatin Recombinant Rabbit Polyclonal Antibody (Product # 710115) at a dilution of 1 µg/mL.