Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [6]
- Immunocytochemistry [2]
- Immunohistochemistry [1]
- Other assay [1]
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- Product number
- PA5-85532 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- NAMPT Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant full-length protein
- Description
- For reconstitution, we recommend adding 100 µL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiments, we strongly recommend performing another round of desalting. (Zeba Spin Desalting Columns, 7KMWCO, 0.5 mL, Product # 89882)
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µL
- Concentration
- 1 mg/mL
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of NAMPT in A) NCI-H929 whole cell lysate using NAMPT polyclonal antibody (Product # PA5-85532) at a dilution of 1:1000. Prior to incubation with primary antibody, the sample was separated on 10% SDS-PAGE.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot using NAMPT Polyclonal Antibody (Product # PA5-85532). Various whole cell extracts (30 µg) were separated by 10% SDS-PAGE, and the membrane was blotted with NAMPT Polyclonal Antibody (Product # PA5-85532) diluted at 1:500.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of NAMPT in whole cell extract using NAMPT polyclonal antibody (Product # PA5-85532) using 30 µg of sample at a dilution of 1:500. Prior to incubation with primary antibody, the sample was separated on 10% SDS-PAGE.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot using NAMPT Polyclonal Antibody (Product # PA5-85532). Mouse tissue extract (50 µg) was separated by 10% SDS-PAGE, and the membrane was blotted with NAMPT Polyclonal Antibody (Product # PA5-85532) diluted at 1:500.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of NAMPT in various whole cell extracts using NAMPT polyclonal antibody (Product # PA5-85532) using 30 µg of sample at a dilution of 1:500. Prior to incubation with primary antibody, the sample was separated on 10% SDS-PAGE.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- NAMPT antibody detects Visfatin protein by western blot analysis. A NCI-H929 whole cell lysate/extract.10 % SDS-PAGE. NAMPT Polyclonal Antibody (Product # PA5-85532) dilution: 1:1,000.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunocytochemistry analysis of NAMPT in 293T cells using NAMPT polyclonal antibody (Product # PA5-85532) at a dilution of 1:500. Sample was then incubated with Hoechst secondary antibody.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- NAMPT Polyclonal Antibody detects Visfatin protein at cytoplasm and nucleus by immunofluorescent analysis. Sample: 293T cells were fixed in ice-cold MeOH for 5 min. Green: Visfatin protein stained by NAMPT Polyclonal Antibody (Product # PA5-85532) diluted at 1:500. Blue: Hoechst 33342 staining. Scale bar = 10 µm.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- NAMPT Polyclonal Antibody detects Visfatin protein at cytosol and nucleus on mouse muscle by immunohistochemical analysis. Sample: Paraffin-embedded mouse muscle. NAMPT Polyclonal Antibody (Product # PA5-85532) dilution: 1:500. Antigen Retrieval: EDTA based buffer, pH 8.0, 15 min.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunoprecipitation analysis of NAMPT in 293T whole cell extracts with NAMPT polyclonal antibody (Product # PA5-85532) using 5 µg of sample, followed by anti-Rabbit IgG secondary antibody.