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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [7]
- Immunocytochemistry [1]
- Immunohistochemistry [1]
- Other assay [2]
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- Product number
- PA5-85535 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- NAMPT Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant full-length protein
- Description
- Keep as concentrated solution.
- Concentration
- 1 mg/mL
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of NAMPT in various whole cell extracts using NAMPT polyclonal antibody (Product # PA5-85535) using 30 µg of sample at a dilution of 1:500. Sample was then incubated with HRP-conjugated anti-rabbit IgG secondary antibody. Prior to incubation with primary antibody, the sample was separated on 10% SDS-PAGE.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of NAMPT in various whole cell extracts using NAMPT polyclonal antibody (Product # PA5-85535) using 30 µg of sample at a dilution of 1:500. Sample was then incubated with HRP-conjugated anti-rabbit IgG secondary antibody. Prior to incubation with primary antibody, the sample was separated on 10% SDS-PAGE.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of NAMPT in A) K562 whole cell lysate using NAMPT polyclonal antibody (Product # PA5-85535) using 30 µg of sample at a dilution of 1:1000. Sample was then incubated with HRP-conjugated anti-rabbit IgG secondary antibody. Prior to incubation with primary antibody, the sample was separated on 10% SDS-PAGE.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot using NAMPT Polyclonal Antibody (Product # PA5-85535). Various whole cell extracts (30 µg) were separated by 10% SDS-PAGE, and the membrane was blotted with NAMPT Polyclonal Antibody (Product # PA5-85535) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot using NAMPT Polyclonal Antibody (Product # PA5-85535). Various whole cell extracts (30 µg) were separated by 10% SDS-PAGE, and the membrane was blotted with NAMPT Polyclonal Antibody (Product # PA5-85535) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- PBEF antibody detects Visfatin protein by western blot analysis. A. 30 µg 293T whole cell lysate/extract. B. 30 µg A431 whole cell lysate/extract.10% SDS-PAGE. NAMPT Polyclonal Antibody (Product # PA5-85535) dilution: 1:1,000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- PBEF antibody detects Visfatin protein by western blot analysis. A. 30 µg K562 whole cell lysate/extract.10% SDS-PAGE. NAMPT Polyclonal Antibody (Product # PA5-85535) dilution: 1:1,000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- NAMPT Polyclonal Antibody detects Visfatin protein at cytoplasm by immunofluorescent analysis. Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: Visfatin protein stained by NAMPT Polyclonal Antibody (Product # PA5-85535) diluted at 1:500. Blue: Hoechst 33342 staining.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- PBEF antibody detects Visfatin protein at cytosol on human hepatoma by immunohistochemical analysis. Sample: Paraffin-embedded hepatoma. NAMPT Polyclonal Antibody (Product # PA5-85535) dilution: 1:500. Antigen Retrieval: EDTA based buffer, pH 8.0, 15 min.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunoprecipitation analysis of NAMPT in 293T whole cell extracts with NAMPT polyclonal antibody (Product # PA5-85535) using 5 µg of sample, followed by anti-Rabbit IgG secondary antibody.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunoprecipitation analysis of NAMPT in 293T whole cell extracts with NAMPT polyclonal antibody (Product # PA5-85535) using 5 µg of sample, followed by anti-Rabbit IgG secondary antibody.
