PA5-23198

antibody from Invitrogen Antibodies

Targeting: NAMPT PBEF, PBEF1

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Data presented on provider website)

Antibody Data

Product number

PA5-23198

Provider

Invitrogen Antibodies

Product name

Anti-NAMPT Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Synthetic peptide

Reactivity

Human, Mouse, Rat, Chicken/Avian

Host

Rabbit

Isotype

IgG

Vial size

100 µL

Concentration

Conc. Not Determined

Storage

-20° C, Avoid Freeze/Thaw Cycles

References

Submitted references

Developmental cigarette smoke exposure II: Hippocampus proteome and metabolome profiles in adult offspring.

Neal RE, Jagadapillai R, Chen J, Webb C, Stocke K, Greene RM, Pisano MM
Reproductive toxicology (Elmsford, N.Y.) 2016 Oct;65:436-447

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis of NAMPT/Visfatin in skeletal muscle lysate. Lane 1 shows pre-immune sera. Lane 2 shows human, Lane 3 shows mouse and Lane 4 shows rat skeletal muscle using a NAMPT/Visfatin polyclonal antibody (Product # PA5-23198) at 1:5000 dilution.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis was performed on whole cell extracts (30 µg lysate) of U-87 MG (Lane 1), PC-3 (Lane 2), A-431 (Lane 3), HCT 116 (Lane 4), Jurkat (Lane 5), Raji (Lane 6), MCF7 (Lane 7), A549 (Lane 8) and Mouse Brain (Lane 9). The blot was probed with Anti-NAMPT Polyclonal Antibody (Product # PA5-23198, 1:2500 dilution) and detected by chemiluminescence using Goat anti-Rabbit IgG (H+L) Superclonal™ Secondary Antibody, HRP conjugate (Product # A27036, 0.25 µg/mL, 1:4000 dilution). A 56kDa band corresponding to NAMPT was observed across all the cell lines and tissue lysates tested. Uncharacterized bands were observed at ~60kDa.