Antibody data
- Antibody Data
- Antigen structure
- References [2]
- Comments [0]
- Validations
- Western blot [1]
- Flow cytometry [1]
- Blocking/Neutralizing [1]
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Validation data
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- Product number
- NBP2-27338 - Provider product page
- Provider
- Novus Biologicals
- Product name
- Mouse Monoclonal IL-17/IL-17A Antibody
- Antibody type
- Monoclonal
- Description
- Protein G purified.
- Reactivity
- Human
- Host
- Mouse
- Isotype
- IgG
- Vial size
- 0.1 mg
- Concentration
- 0.5 mg/ml
- Storage
- Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Submitted references Effects of interleukin-17 on human retinal vascular endothelial cell capillary tube formation in vitro.
Potassium supplementation inhibits IL-17A production induced by salt loading in human T lymphocytes via p38/MAPK-SGK1 pathway.
Liu G, Wu H, Chen L, Xu J, Wang M, Li D, Lu P
Molecular medicine reports 2017 Jul;16(1):865-872
Molecular medicine reports 2017 Jul;16(1):865-872
Potassium supplementation inhibits IL-17A production induced by salt loading in human T lymphocytes via p38/MAPK-SGK1 pathway.
Wen W, Wan Z, Ren K, Zhou D, Gao Q, Wu Y, Wang L, Yuan Z, Zhou J
Experimental and molecular pathology 2016 Jun;100(3):370-7
Experimental and molecular pathology 2016 Jun;100(3):370-7
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: IL-17/IL-17A Antibody (4H1524) [NBP2-27338] - Analysis of IL-17A in 1) human, 2) mouse and 3) rat full-length recombinant IL-17A protein using NBP2-27338 at 1 ug/ml.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Flow Cytometry: IL-17/IL-17A Antibody (4H1524) [NBP2-27338] - Analysis using the PE conjugate of NBP2-25229. IL-17A positive cells in PMA/IM stimulated fresh PBMC: PBMC stimulated for 6h with PMA (25ng/ml) & IM (250ng/ml), in the presence of BFA. Cells were surface stained with CD4 APC, fixed & permeabilised for intracellular staining with PE conjugated
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Block/Neutralize: IL-17/IL-17A Antibody (4H1524) [NBP2-27338] - Analysis using Azide Free version of NBP2-27338. Neutralization by Human IL-17A Antibody. Secretion of IL-6 by 3T3 cells stimulated with recombinant human IL-17A (20ng/ml) is neutralized by increasing concentrations of IL-17A monoclonal antibody. Antibody