Antibody data
- Antibody Data
- Antigen structure
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- Validations
- Other assay [1]
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- Product number
- MA1069B - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- IL-17A Monoclonal Antibody (3.41.2.6.1), Biotin
- Antibody type
- Monoclonal
- Antigen
- Other
- Description
- The MA1068 anti-IL17a antibody (clone 3-36.2.7.3) has successfully been paired as the coating antibody in a sandwich ELISA with detection antibody MA1069B (biotinylated conjugate of clone 3-41.2.6.1). Typical dilutions for sandwich ELISA include 1 µg/mL for coating and 0.125 - 0.25 µg/mL for detection.
- Reactivity
- Human
- Host
- Mouse
- Conjugate
- Biotin
- Isotype
- IgG
- Antibody clone number
- 3.41.2.6.1
- Vial size
- 100 µg
- Concentration
- 0.5 mg/mL
- Storage
- -20° C, Avoid Freeze/Thaw Cycles
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Supportive validation
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- Invitrogen Antibodies (provider)
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- Experimental details
- Sandwich ELISA analysis of Human IL-17A monoclonal antibody was performed using the Thermo Scientific ELISA Kit (Product # EHIL17A) by coating a blank 96-well microtiter plate with 100 µL per well of Human IL-17A moncolonal antibody (Product # MA1068) in duplicate at 1, 3, 4, 5, 7, and 9 µg/mL in DPBS (Product # 28374) and incubating for 12-18 hours at 4C. The plate was aspirated and blocked with 300 µL per well of 4% BSA and 5% sucrose in DPBS for 1 hour at room temperature. Human IL-17A recombinant protein at 50 µL per well was added in duplicate at 2000, 1000, 500, 250, 125, 62.5, 31.25, and 0 pg/mL for 2 hours at room temperature along with 50 µL of Human IL-17A biotinylated monoclonal antibody (Product # MA1069B) in all applicable wells at 0.1 µg/mL for 2 hours at room temperature. The plate was washed with ELISA Wash Buffer (Product # N503) and incubated with 100 µL per well of Streptavidin-HRP (Product # N504) in all test wells at 1:40,000 dilution for 30 minutes at room temperature and then washed and incubated with 100 µL per well of TMB substrate (Product # 34028) for 30 minutes at room temperature in the dark. The plate was stopped with 0.16M sulfuric acid (Product # N600). Absorbances were read on a spectrophotometer at 450-550 nm.
- Conjugate
- Biotin