Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [1]
- Flow cytometry [1]
- Blocking/Neutralizing [1]
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Validation data
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- Product number
- NBP2-25229 - Provider product page
- Provider
- Novus Biologicals
- Product name
- Mouse Monoclonal IL-17/IL-17A Antibody
- Antibody type
- Monoclonal
- Description
- Protein G purified.
- Reactivity
- Human
- Host
- Mouse
- Isotype
- IgG
- Vial size
- 0.25 mg
- Storage
- Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: IL-17/IL-17A Antibody (4H1524) - Azide Free [NBP2-25229] - Analysis of IL-17A in (1) human, (2) mouse and (3) rat full-length recombinant IL-17A protein using NBP2-25229 at 1 ug/ml.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Flow Cytometry: IL-17/IL-17A Antibody (4H1524) - Azide Free [NBP2-25229] - Analysis using the PE conjugate of NBP2-25229. IL-17A positive cells in PMA/IM stimulated fresh PBMC: PBMC stimulated for 6h with PMA (25ng/ml) & IM (250ng/ml), in the presence of BFA. Cells were surface stained with CD4 APC, fixed & permeabilizedfor intracellular staining with PE conjugated
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Block/Neutralize: IL-17/IL-17A Antibody (4H1524) - Azide Free [NBP2-25229] - Neutralization by Human IL-17A Antibody. Secretion of IL-6 by 3T3 cells stimulated with recombinant human IL-17A (20ng/ml) is neutralized by increasing concentrations of IL-17A monoclonal antibody. Antibody at different concentrations was added to cells 1hr prior to addition of cytokine. Supernatant collected from 60hr cultures was used for analysis to measure secreted IL-6 by ELISA.