IC317P

antibody from Novus Biologicals

Targeting: IL17A CTLA8, IL-17, IL-17A, IL17

Flow cytometry (Supportive data in Antibodypedia)

Antibody Data

Product number

IC317P

Provider

Novus Biologicals

Product name

Mouse Monoclonal IL-17/IL-17A Antibody

Antibody type

Monoclonal

Description

Protein A or G purified from ascites. Detects human IL-17/IL-17A in direct ELISAs. In direct ELISAs, approximately 12% cross-reactivity with recombinant canine IL-17/IL-17A is observed and 25%-50% reactivity with recombinant human (rh) IL-17A/IL-17F heterodimer is observed. No cross-reactivity with recombinant mouse IL-17/IL-17A, rhIL-17B, rhIL-17C, rhIL-17D, rhIL-17E, or rhIL-17F is observed.

Reactivity

Human

Host

Mouse

Isotype

IgG

Vial size

100 Tests

Storage

Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 degreesC as supplied.

References

Submitted references

Enhancing the toolbox to study IL-17A in cattle and sheep.

Wattegedera SR, Corripio-Miyar Y, Pang Y, Frew D, McNeilly TN, Palarea-Albaladejo J, McInnes CJ, Hope JC, Glass EJ, Entrican G
Veterinary research 2017 Apr 8;48(1):20

Flow cytometry

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Detection of IL-17/IL-17A in Th17-differentiated Human CD4⁺ PBMCs by Flow Cytometry. Human CD4⁺ peripheral blood mononuclear cells (PBMCs) either (A) untreated or (B) treated with 50 ng/mL PMA, 200 ng/mL Calcium Ionomycin, 200 ng/mL LPS, 20 ng/mL Recombinant Human IL-23 (Catalog # 1290-IL), and 40 ng/mL Recombinant Human IL-6 (Catalog # 206-IL) overnight to induce Th17 development were stained with Mouse Anti-Human IL-17/IL-17A PE-conjugated Monoclonal Antibody (Catalog # IC317P) and Mouse Anti-Human CD3 epsilon APC-conjugated Monoclonal Antibody (Catalog # FAB100A). Quadrant markers were set based on control antibody staining (Catalog # IC0041P). To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005). View our protocol for Staining Intracellular Molecules.