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- Antibody Data
- Antigen structure
- References [13]
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- Blocking/Neutralizing [1]
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- Product number
- AF-421-NA - Provider product page
- Provider
- Novus Biologicals
- Product name
- Goat Polyclonal IL-17/IL-17A Antibody
- Antibody type
- Polyclonal
- Description
- Immunogen affinity purified. Detects mouse and rat IL-17 in direct ELISAs and Western blots. In direct ELISAs and Western blots, approximately 10% cross-reactivity with recombinant human IL-17A and recombinant mouse (rm) IL-17F is observed and less than 1% cross-reactivity with rmIL-17B, rmIL-17C, rmIL-17D, and rmIL-17E is observed.
- Reactivity
- Mouse
- Host
- Goat
- Isotype
- IgG
- Vial size
- 100 ug
- Concentration
- LYOPH
- Storage
- Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 degreesC as supplied. 1 month, 2 to 8 degreesC under sterile conditions after reconstitution. 6 months, -20 to -70 degreesC under sterile conditions after reconstitution.
Submitted references IL-33 Signaling Regulates Innate IL-17A and IL-22 Production via Suppression of Prostaglandin E(2) during Lung Fungal Infection.
IL17 Promotes Mammary Tumor Progression by Changing the Behavior of Tumor Cells and Eliciting Tumorigenic Neutrophils Recruitment.
IL-17 and VEGF are necessary for efficient corneal nerve regeneration.
Microenvironment-derived IL-1 and IL-17 interact in the control of lung metastasis.
Anti-inflammatory effects of IL-17A on Helicobacter pylori-induced gastritis.
Targeting Tim-1 to overcome resistance to transplantation tolerance mediated by CD8 T17 cells.
Prostaglandin mediates IL-23/IL-17-induced neutrophil migration in inflammation by inhibiting IL-12 and IFNgamma production.
Resveratrol inhibits high glucose-induced PI3K/Akt/ERK-dependent interleukin-17 expression in primary mouse cardiac fibroblasts.
Tumor-specific Th17-polarized cells eradicate large established melanoma.
T-bet regulates the fate of Th1 and Th17 lymphocytes in autoimmunity.
T-bet regulates the fate of Th1 and Th17 lymphocytes in autoimmunity.
IL-17, produced by lymphocytes and neutrophils, is necessary for lipopolysaccharide-induced airway neutrophilia: IL-15 as a possible trigger.
IL-17, produced by lymphocytes and neutrophils, is necessary for lipopolysaccharide-induced airway neutrophilia: IL-15 as a possible trigger.
Garth JM, Reeder KM, Godwin MS, Mackel JJ, Dunaway CW, Blackburn JP, Steele C
Journal of immunology (Baltimore, Md. : 1950) 2017 Sep 15;199(6):2140-2148
Journal of immunology (Baltimore, Md. : 1950) 2017 Sep 15;199(6):2140-2148
IL17 Promotes Mammary Tumor Progression by Changing the Behavior of Tumor Cells and Eliciting Tumorigenic Neutrophils Recruitment.
Benevides L, da Fonseca DM, Donate PB, Tiezzi DG, De Carvalho DD, de Andrade JM, Martins GA, Silva JS
Cancer research 2015 Sep 15;75(18):3788-99
Cancer research 2015 Sep 15;75(18):3788-99
IL-17 and VEGF are necessary for efficient corneal nerve regeneration.
Li Z, Burns AR, Han L, Rumbaut RE, Smith CW
The American journal of pathology 2011 Mar;178(3):1106-16
The American journal of pathology 2011 Mar;178(3):1106-16
Microenvironment-derived IL-1 and IL-17 interact in the control of lung metastasis.
Carmi Y, Rinott G, Dotan S, Elkabets M, Rider P, Voronov E, Apte RN
Journal of immunology (Baltimore, Md. : 1950) 2011 Mar 15;186(6):3462-71
Journal of immunology (Baltimore, Md. : 1950) 2011 Mar 15;186(6):3462-71
Anti-inflammatory effects of IL-17A on Helicobacter pylori-induced gastritis.
Otani K, Watanabe T, Tanigawa T, Okazaki H, Yamagami H, Watanabe K, Tominaga K, Fujiwara Y, Oshitani N, Arakawa T
Biochemical and biophysical research communications 2009 May 1;382(2):252-8
Biochemical and biophysical research communications 2009 May 1;382(2):252-8
Targeting Tim-1 to overcome resistance to transplantation tolerance mediated by CD8 T17 cells.
Yuan X, Ansari MJ, D'Addio F, Paez-Cortez J, Schmitt I, Donnarumma M, Boenisch O, Zhao X, Popoola J, Clarkson MR, Yagita H, Akiba H, Freeman GJ, Iacomini J, Turka LA, Glimcher LH, Sayegh MH
Proceedings of the National Academy of Sciences of the United States of America 2009 Jun 30;106(26):10734-9
Proceedings of the National Academy of Sciences of the United States of America 2009 Jun 30;106(26):10734-9
Prostaglandin mediates IL-23/IL-17-induced neutrophil migration in inflammation by inhibiting IL-12 and IFNgamma production.
Lemos HP, Grespan R, Vieira SM, Cunha TM, Verri WA Jr, Fernandes KS, Souto FO, McInnes IB, Ferreira SH, Liew FY, Cunha FQ
Proceedings of the National Academy of Sciences of the United States of America 2009 Apr 7;106(14):5954-9
Proceedings of the National Academy of Sciences of the United States of America 2009 Apr 7;106(14):5954-9
Resveratrol inhibits high glucose-induced PI3K/Akt/ERK-dependent interleukin-17 expression in primary mouse cardiac fibroblasts.
Venkatachalam K, Mummidi S, Cortez DM, Prabhu SD, Valente AJ, Chandrasekar B
American journal of physiology. Heart and circulatory physiology 2008 May;294(5):H2078-87
American journal of physiology. Heart and circulatory physiology 2008 May;294(5):H2078-87
Tumor-specific Th17-polarized cells eradicate large established melanoma.
Muranski P, Boni A, Antony PA, Cassard L, Irvine KR, Kaiser A, Paulos CM, Palmer DC, Touloukian CE, Ptak K, Gattinoni L, Wrzesinski C, Hinrichs CS, Kerstann KW, Feigenbaum L, Chan CC, Restifo NP
Blood 2008 Jul 15;112(2):362-73
Blood 2008 Jul 15;112(2):362-73
T-bet regulates the fate of Th1 and Th17 lymphocytes in autoimmunity.
Gocke AR, Cravens PD, Ben LH, Hussain RZ, Northrop SC, Racke MK, Lovett-Racke AE
Journal of immunology (Baltimore, Md. : 1950) 2007 Feb 1;178(3):1341-8
Journal of immunology (Baltimore, Md. : 1950) 2007 Feb 1;178(3):1341-8
T-bet regulates the fate of Th1 and Th17 lymphocytes in autoimmunity.
Gocke AR, Cravens PD, Ben LH, Hussain RZ, Northrop SC, Racke MK, Lovett-Racke AE
Journal of immunology (Baltimore, Md. : 1950) 2007 Feb 1;178(3):1341-8
Journal of immunology (Baltimore, Md. : 1950) 2007 Feb 1;178(3):1341-8
IL-17, produced by lymphocytes and neutrophils, is necessary for lipopolysaccharide-induced airway neutrophilia: IL-15 as a possible trigger.
Ferretti S, Bonneau O, Dubois GR, Jones CE, Trifilieff A
Journal of immunology (Baltimore, Md. : 1950) 2003 Feb 15;170(4):2106-12
Journal of immunology (Baltimore, Md. : 1950) 2003 Feb 15;170(4):2106-12
IL-17, produced by lymphocytes and neutrophils, is necessary for lipopolysaccharide-induced airway neutrophilia: IL-15 as a possible trigger.
Ferretti S, Bonneau O, Dubois GR, Jones CE, Trifilieff A
Journal of immunology (Baltimore, Md. : 1950) 2003 Feb 15;170(4):2106-12
Journal of immunology (Baltimore, Md. : 1950) 2003 Feb 15;170(4):2106-12
No comments: Submit comment
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Detection of IL-17 in EL-4 Mouse Cell Line by Flow Cytometry. EL-4 mouse lymphoblast cell line was treated for 16 hours with 50 ng/mL PMA then stained with Goat Anti-Mouse IL-17 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-421-NA, filled histogram) or isotype control antibody (Catalog # AB-108-C, open histogram), followed by Phycoerythrin-conjugated Anti-Goat IgG Secondary Antibody (Catalog # F0107). To facilitate intracellular staining, cells were fixed with paraformaldehyde and permeabilized with saponin.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- IL-6 Secretion Induced by IL-17 and Neutralization by Mouse IL-17 Antibody. Recombinant Mouse IL-17 (Catalog # 421-ML) stimulates IL-6 secretion in the NIH-3T3 mouse embryonic fibroblast cell line in a dose-dependent manner (orange line), as measured by the Mouse IL-6 Quantikine ELISA Kit (Catalog # M6000B). IL-6 secretion elicited by Recombinant Mouse IL-17 (10 ng/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Mouse IL-17 Antigen Affinity-purified Polyclonal Anti-body (Catalog # AF-421-NA). The ND50 is typically 0.05-0.25 µg/mL.
