IC3171G-100

antibody from Novus Biologicals

Targeting: IL17A CTLA8, IL-17, IL-17A, IL17

Flow cytometry (Supportive data in Antibodypedia)

Antibody Data

Product number

IC3171G-100

Provider

Novus Biologicals

Product name

Mouse Monoclonal IL-17/IL-17A Antibody

Antibody type

Monoclonal

Description

Protein A or G purified from hybridoma culture supernatant. Detects human IL-17/IL-17A in direct ELISAs. In direct ELISAs, approximately 40% cross-reactivity with recombinant human (rh) IL-17A/IL-17F heterodimer is observed. No cross-reactivity with recombinant mouse IL-17/IL-17A, recombinant canine IL-17/IL-17A, rhIL-17B, rhIL-17C, rhIL-17D, rhIL-17E, or rhIL-17F is observed.

Reactivity

Human

Host

Mouse

Conjugate

Green dye

Isotype

IgG

Vial size

100 Tests

Storage

Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 degreesC as supplied.

Flow cytometry

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Detection of IL-17/IL-17A in Human PBMCs by Flow Cytometry. Human peripheral blood mononuclear cells (PBMCs) treated with PMA, Calcium Ionomycin, LPS, and Recombinant Human IL-23 (Catalog # 1290-IL) to induce Th17 development overnight, re-stimulated with PMA, Calcium Ionomycin, and Monensin for 2 hours were stained with Mouse Anti-Human IL-17/IL-17A Alexa Fluor® 488-conjugated Monoclonal Antibody (Catalog # IC3171G, filled histogram) or isotype control antibody (Catalog # IC002G, open histogram). To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005). Histograms were gated on CD4⁺ cells. View our protocol for Staining Intracellular Molecules.