Antibody data
- Antibody Data
- Antigen structure
- References [16]
- Comments [0]
- Validations
- Western blot [1]
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- Product number
- MAB721-100 - Provider product page
- Provider
- R&D Systems
- Product name
- Mouse IL-17/IL-17A Antibody
- Antibody type
- Monoclonal
- Description
- Protein A or G purified from hybridoma culture supernatant. Detects mouse IL-17 in ELISAs and Western blots. In direct ELISAs, 100% reactivity with recombinant mouse (rm) IL-17A/IL-17F heterodimer, 50% cross-reactivity with recombinant human IL-17, and 25% cross-reactivity with recombinant canine IL-17 is observed. No cross-reactivity with rmIL-17B, rmIL-17C, rmIL-17D, rmIL-17E, or rmIL-17F is observed. In Western blots, cross-reactivity with recombinant rat IL-17A is observed.
- Reactivity
- Mouse
- Host
- Rat
- Conjugate
- Unconjugated
- Antigen sequence
Q62386
- Isotype
- IgG
- Antibody clone number
- 50101
- Vial size
- 100 ug
- Storage
- Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Submitted references IL-33 Attenuates Sepsis by Inhibiting IL-17 Receptor Signaling through Upregulation of SOCS3.
The Major Histocompatibility Complex Class III Haplotype Ltab-Ncr3 Regulates Adjuvant-Induced but Not Antigen-Induced Autoimmunity.
MHC class II alleles associated with Th1 rather than Th17 type immunity drive the onset of early arthritis in a rat model of rheumatoid arthritis.
Interferon-gamma regulates inflammatory cell death by targeting necroptosis in experimental autoimmune arthritis.
Enhanced immunoregulation of mesenchymal stem cells by IL-10-producing type 1 regulatory T cells in collagen-induced arthritis.
Targeting dendritic cells to accelerate T-cell activation overcomes a bottleneck in tuberculosis vaccine efficacy.
CD8⁺ cells regulate the T helper-17 response in an experimental murine model of Sjögren syndrome.
Interferon gamma suppresses collagen-induced arthritis by regulation of Th17 through the induction of indoleamine-2,3-deoxygenase.
Response patterns of cytokines/chemokines in two murine strains after irradiation.
CCL25 induces α₄β₇ integrin-dependent migration of IL-17⁺ γδ T lymphocytes during an allergic reaction.
Prostaglandin mediates IL-23/IL-17-induced neutrophil migration in inflammation by inhibiting IL-12 and IFNgamma production.
Tumor-specific Th17-polarized cells eradicate large established melanoma.
The proinflammatory effect of prostaglandin E2 in experimental inflammatory bowel disease is mediated through the IL-23-->IL-17 axis.
IL-17 production is dominated by gammadelta T cells rather than CD4 T cells during Mycobacterium tuberculosis infection.
IL-23 compensates for the absence of IL-12p70 and is essential for the IL-17 response during tuberculosis but is dispensable for protection and antigen-specific IFN-gamma responses if IL-12p70 is available.
IL-1-independent role of IL-17 in synovial inflammation and joint destruction during collagen-induced arthritis.
Lv R, Zhao J, Lei M, Xiao D, Yu Y, Xie J
Cellular physiology and biochemistry : international journal of experimental cellular physiology, biochemistry, and pharmacology 2017;42(5):1961-1972
Cellular physiology and biochemistry : international journal of experimental cellular physiology, biochemistry, and pharmacology 2017;42(5):1961-1972
The Major Histocompatibility Complex Class III Haplotype Ltab-Ncr3 Regulates Adjuvant-Induced but Not Antigen-Induced Autoimmunity.
Yau ACY, Tuncel J, Holmdahl R
The American journal of pathology 2017 May;187(5):987-998
The American journal of pathology 2017 May;187(5):987-998
MHC class II alleles associated with Th1 rather than Th17 type immunity drive the onset of early arthritis in a rat model of rheumatoid arthritis.
Tuncel J, Haag S, Holmdahl R
European journal of immunology 2017 Mar;47(3):563-574
European journal of immunology 2017 Mar;47(3):563-574
Interferon-gamma regulates inflammatory cell death by targeting necroptosis in experimental autoimmune arthritis.
Lee SH, Kwon JY, Kim SY, Jung K, Cho ML
Scientific reports 2017 Aug 31;7(1):10133
Scientific reports 2017 Aug 31;7(1):10133
Enhanced immunoregulation of mesenchymal stem cells by IL-10-producing type 1 regulatory T cells in collagen-induced arthritis.
Lim JY, Im KI, Lee ES, Kim N, Nam YS, Jeon YW, Cho SG
Scientific reports 2016 Jun 1;6:26851
Scientific reports 2016 Jun 1;6:26851
Targeting dendritic cells to accelerate T-cell activation overcomes a bottleneck in tuberculosis vaccine efficacy.
Griffiths KL, Ahmed M, Das S, Gopal R, Horne W, Connell TD, Moynihan KD, Kolls JK, Irvine DJ, Artyomov MN, Rangel-Moreno J, Khader SA
Nature communications 2016 Dec 22;7:13894
Nature communications 2016 Dec 22;7:13894
CD8⁺ cells regulate the T helper-17 response in an experimental murine model of Sjögren syndrome.
Zhang X, Schaumburg CS, Coursey TG, Siemasko KF, Volpe EA, Gandhi NB, Li DQ, Niederkorn JY, Stern ME, Pflugfelder SC, de Paiva CS
Mucosal immunology 2014 Mar;7(2):417-27
Mucosal immunology 2014 Mar;7(2):417-27
Interferon gamma suppresses collagen-induced arthritis by regulation of Th17 through the induction of indoleamine-2,3-deoxygenase.
Lee J, Lee J, Park MK, Lim MA, Park EM, Kim EK, Yang EJ, Lee SY, Jhun JY, Park SH, Kim HY, Cho ML
PloS one 2013;8(4):e60900
PloS one 2013;8(4):e60900
Response patterns of cytokines/chemokines in two murine strains after irradiation.
Zhang M, Yin L, Zhang K, Sun W, Yang S, Zhang B, Salzman P, Wang W, Liu C, Vidyasagar S, Zhang L, Ju S, Okunieff P, Zhang L
Cytokine 2012 May;58(2):169-77
Cytokine 2012 May;58(2):169-77
CCL25 induces α₄β₇ integrin-dependent migration of IL-17⁺ γδ T lymphocytes during an allergic reaction.
Costa MF, Bornstein VU, Candéa AL, Henriques-Pons A, Henriques MG, Penido C
European journal of immunology 2012 May;42(5):1250-60
European journal of immunology 2012 May;42(5):1250-60
Prostaglandin mediates IL-23/IL-17-induced neutrophil migration in inflammation by inhibiting IL-12 and IFNgamma production.
Lemos HP, Grespan R, Vieira SM, Cunha TM, Verri WA Jr, Fernandes KS, Souto FO, McInnes IB, Ferreira SH, Liew FY, Cunha FQ
Proceedings of the National Academy of Sciences of the United States of America 2009 Apr 7;106(14):5954-9
Proceedings of the National Academy of Sciences of the United States of America 2009 Apr 7;106(14):5954-9
Tumor-specific Th17-polarized cells eradicate large established melanoma.
Muranski P, Boni A, Antony PA, Cassard L, Irvine KR, Kaiser A, Paulos CM, Palmer DC, Touloukian CE, Ptak K, Gattinoni L, Wrzesinski C, Hinrichs CS, Kerstann KW, Feigenbaum L, Chan CC, Restifo NP
Blood 2008 Jul 15;112(2):362-73
Blood 2008 Jul 15;112(2):362-73
The proinflammatory effect of prostaglandin E2 in experimental inflammatory bowel disease is mediated through the IL-23-->IL-17 axis.
Sheibanie AF, Yen JH, Khayrullina T, Emig F, Zhang M, Tuma R, Ganea D
Journal of immunology (Baltimore, Md. : 1950) 2007 Jun 15;178(12):8138-47
Journal of immunology (Baltimore, Md. : 1950) 2007 Jun 15;178(12):8138-47
IL-17 production is dominated by gammadelta T cells rather than CD4 T cells during Mycobacterium tuberculosis infection.
Lockhart E, Green AM, Flynn JL
Journal of immunology (Baltimore, Md. : 1950) 2006 Oct 1;177(7):4662-9
Journal of immunology (Baltimore, Md. : 1950) 2006 Oct 1;177(7):4662-9
IL-23 compensates for the absence of IL-12p70 and is essential for the IL-17 response during tuberculosis but is dispensable for protection and antigen-specific IFN-gamma responses if IL-12p70 is available.
Khader SA, Pearl JE, Sakamoto K, Gilmartin L, Bell GK, Jelley-Gibbs DM, Ghilardi N, deSauvage F, Cooper AM
Journal of immunology (Baltimore, Md. : 1950) 2005 Jul 15;175(2):788-95
Journal of immunology (Baltimore, Md. : 1950) 2005 Jul 15;175(2):788-95
IL-1-independent role of IL-17 in synovial inflammation and joint destruction during collagen-induced arthritis.
Lubberts E, Joosten LA, Oppers B, van den Bersselaar L, Coenen-de Roo CJ, Kolls JK, Schwarzenberger P, van de Loo FA, van den Berg WB
Journal of immunology (Baltimore, Md. : 1950) 2001 Jul 15;167(2):1004-13
Journal of immunology (Baltimore, Md. : 1950) 2001 Jul 15;167(2):1004-13
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Supportive validation
- Submitted by
- R&D Systems (provider)
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- Experimental details
- Detection of Recombinant Mouse and Rat IL-17/IL-17A by Western Blot. Western blot shows 25 ng of Recombinant Mouse IL-17/IL-17A (Catalog # 421-ML), Recombinant Human IL-17/IL-17A (Catalog # 317-ILB), Recombinant Rat IL-17/IL-17A (Catalog # 8410-IL), and Recombinant Mouse IL-17F (Catalog # 2057-IL). PVDF Membrane was probed with 1 µg/mL of Rat Anti-Mouse IL-17/IL-17A Monoclonal Antibody (Catalog # MAB721) followed by HRP-conjugated Anti-Rat IgG Secondary Antibody (Catalog # HAF005). A specific band was detected for IL-17/IL-17A at approximately 14 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 3.