Antibody data
- Antibody Data
- Antigen structure
- References [5]
- Comments [0]
- Validations
- Flow cytometry [1]
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- Product number
- IC3171P-100 - Provider product page
- Provider
- R&D Systems
- Product name
- Human IL-17/IL-17A PE-conjugated Antibody
- Antibody type
- Monoclonal
- Description
- Protein A or G purified from hybridoma culture supernatant. Detects human IL-17 in direct ELISAs. In direct ELISAs, approximately 40% cross-reactivity with recombinant human (rh) IL-17A/IL-17F heterodimer is observed. No cross-reactivity with recombinant mouse IL-17, recombinant canine Il-17, rhIL-17B, rhIL-17C, rhIL-17D, rhIL-17E, or rhIL-17F is observed.
- Reactivity
- Human
- Host
- Mouse
- Antigen sequence
Q16552
- Isotype
- IgG
- Antibody clone number
- 41802
- Vial size
- 100 Tests
- Storage
- Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied.
Submitted references CD4 T-cell expression of IFN-γ and IL-17 in pediatric malarial anemia.
Enhancing the toolbox to study IL-17A in cattle and sheep.
Identification of human NK17/NK1 cells.
Langerhans cell histiocytosis reveals a new IL-17A-dependent pathway of dendritic cell fusion.
Human CD4(+) T cells recognize an epitope within alpha-fetoprotein sequence and develop into TGF-beta-producing CD4(+) T cells.
Raballah E, Kempaiah P, Karim Z, Orinda GO, Otieno MF, Perkins DJ, Ong'echa JM
PloS one 2017;12(4):e0175864
PloS one 2017;12(4):e0175864
Enhancing the toolbox to study IL-17A in cattle and sheep.
Wattegedera SR, Corripio-Miyar Y, Pang Y, Frew D, McNeilly TN, Palarea-Albaladejo J, McInnes CJ, Hope JC, Glass EJ, Entrican G
Veterinary research 2017 Apr 8;48(1):20
Veterinary research 2017 Apr 8;48(1):20
Identification of human NK17/NK1 cells.
Pandya AD, Al-Jaderi Z, Høglund RA, Holmøy T, Harbo HF, Norgauer J, Maghazachi AA
PloS one 2011;6(10):e26780
PloS one 2011;6(10):e26780
Langerhans cell histiocytosis reveals a new IL-17A-dependent pathway of dendritic cell fusion.
Coury F, Annels N, Rivollier A, Olsson S, Santoro A, Speziani C, Azocar O, Flacher M, Djebali S, Tebib J, Brytting M, Egeler RM, Rabourdin-Combe C, Henter JI, Arico M, Delprat C
Nature medicine 2008 Jan;14(1):81-7
Nature medicine 2008 Jan;14(1):81-7
Human CD4(+) T cells recognize an epitope within alpha-fetoprotein sequence and develop into TGF-beta-producing CD4(+) T cells.
Alisa A, Boswell S, Pathan AA, Ayaru L, Williams R, Behboudi S
Journal of immunology (Baltimore, Md. : 1950) 2008 Apr 1;180(7):5109-17
Journal of immunology (Baltimore, Md. : 1950) 2008 Apr 1;180(7):5109-17
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Supportive validation
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- Detection of IL-17/IL-17A in Human PBMCs by Flow Cytometry. Human peripheral blood mononuclear cells (PBMCs) either (A) untreated or (B) treated with PMA and Calcium Ionomycin were stained with Mouse Anti-Human IL-17/IL-17A PE-conjugated Monoclonal Antibody (Catalog # IC3171P) and Mouse Anti-Human CD3 epsilon APC-conjugated Monoclonal Antibody (Catalog # FAB100A). Quadrant markers were set based on control antibody staining (Catalog # IC002P). To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005). View our protocol for Staining Intracellular Molecules.