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Flow cytometryAntibody data
- Antibody Data
- Antigen structure
- References [1]
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- Validations
- Flow cytometry [1]
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- Product number
- IC421C - Provider product page
- Provider
- R&D Systems
- Product name
- Mouse IL-17/IL-17A PerCP-conjugated Antibody
- Antibody type
- Polyclonal
- Description
- Antigen Affinity-purified. Detects mouse IL-17 in direct ELISAs and Western blots. In direct ELISAs and Western blots, approximately 10% cross-reactivity with recombinant human IL-17A and recombinant mouse (rm) IL-17F is observed and less than 1% cross-reactivity with rmIL-17B, rmIL-17C, rmIL-17D, and rmIL-17E is observed.
- Reactivity
- Mouse
- Host
- Goat
- Antigen sequence
Q62386- Isotype
- IgG
- Vial size
- 100 Tests
- Storage
- Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied.
Submitted references Maternal high-fat diet results in microbiota-dependent expansion of ILC3s in mice offspring.
Babu ST, Niu X, Raetz M, Savani RC, Hooper LV, Mirpuri J
JCI insight 2018 Oct 4;3(19)
JCI insight 2018 Oct 4;3(19)
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Supportive validation
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- R&D Systems (provider)
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- Experimental details
- Detection of IL-17/IL-17A in EL-4 Mouse Cell Line by Flow Cytometry. LPS activated EL-4 mouse lymphoblast cell line was stained with Goat Anti-Mouse IL-17/IL-17A PerCP-conjugated Antigen Affinity-purified Polyclonal Antibody (Catalog # IC421C, filled histogram) or isotype control antibody (Catalog # IC108C, open histogram). Cells were fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005). View our protocol for Staining Intracellular Molecules.
