Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [1]
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Validation data
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- Product number
- AP09191PU-S - Provider product page
- Provider
- OriGene
- Product name
- Rabbit Polyclonal Anti-IL17A Antibody, Purified
- Antibody type
- Polyclonal
- Description
- Rabbit Polyclonal Anti-IL17A Antibody, Purified
- Reactivity
- Human
- Host
- Rabbit
- Conjugate
- Unconjugated
- Isotype
- IgG
- Vial size
- 25ul
- Concentration
- 1.0 mg/ml (by UV absorbance at 280 nm)
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Supportive validation
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- OriGene (provider)
- Main image
- Experimental details
- Western blot using anti-Human IL17-A antibody shows detection of a band ~17 kDa in size corresponding to recombinant human IL17-A (lane 1). Molecular weight markers are also shown (M). After transfer, the membrane was blocked overnight with 3% BSA in TBS followed by reaction with primary antibody at a 1:1,000 dilution. Detection occurred using DyLight(TM)649 conjugated anti-Rabbit IgG secondary antibody diluted 1:20,000 in blocking buffer. Image was captured using VersaDoc(TM)MP 4000 imaging system (Bio-Rad).
- Validation comment
- WB