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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [1]
- Flow cytometry [2]
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- Product number
- TA319587 - Provider product page
- Provider
- OriGene
- Product name
- Rat monoclonal Mouse IL-17A antibody
- Antibody type
- Monoclonal
- Description
- Rat monoclonal Mouse IL-17A antibody
- Host
- Rat
- Conjugate
- Unconjugated
- Epitope
- Il17a
- Antibody clone number
- 20B4.G10.F5
- Vial size
- 100 µg
- Concentration
- 2.94mg/ml
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Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- WB showing detection of Mouse IL-17A. 100 ng of Mouse IL-17A was run on a 4-20% gel and transferred to 0.45 ?m nitrocellulose. Anti-Mouse IL-17A (RAT) Antibody (p/n TA319587) was used at 1:1000. Peroxidase conjugated Rabbit Anti-mouse secondary antibody was diluted at 1:40,000 for 30 min at 20?°C and imaged using the Bio-Rad VersaDoc?? 4000 MP. Band indicates correct 23 kDa molecular weight position expected for Mouse IL-17A.
- Validation comment
- WB
Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Anti IL17A was used to detect IL-17A and separate Mouse CD4+ Cells by flow cytometry. Mouse CD4+ T cells were grown on plates coated with anti-CD3 antibody, and stimulated with: 5 ug/mL anti-CD28, 10 ng/mL IL-1beta, 50 ng/mL mouse IL-6, 1 ng/mL TGFbeta1 and 10 ug/mL anti-mouse IFN. Cells were incubated with rat anti-mouse CD4 APC at 0.125 ug/mL, washed, fixed and permeabilized and incubated with Rat anti mouse IL-17A monoclonal Antibody (TA319587) or controls as shown.
- Validation comment
- FC
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Anti IL-17A was used to detect IL-17A and separate Mouse CD4+ Cells by flow cytometry. Mouse CD4+ T cells were grown on plates coated with anti-CD3 antibody, and stimulated with: 5 ug/mL anti-CD28, 10 ng/mL IL-1beta, 50 ng/mL mouse IL-6, 1 ng/mL TGFbeta and 10 ug/mL anti-mouse IFN? over 8-10 days of culture. Cells were incubated with rat anti-mouse CD4 APC at 0.125 ug/mL, washed, fixed and permeabilized and incubated with Rat anti mouse IL-17A monoclonal Antibody (TA319587) or controls as shown.
- Validation comment
- FC
